SciELO - Scientific Electronic Library Online

vol.23 issue3Vibrio cholerae No O1 en muestras de aguas no cloradas consumidas por pobladores de las localidades de Santa y Coishco (Ancash), 2003 - 2004Efecto neutralizador del extracto acuoso de Dracontium loretense (JERGÓN SACHA) sobre la actividad letal del veneno de Bothrops atrox author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO


Revista Peruana de Medicina Experimental y Salud Publica

Print version ISSN 1726-4634


INCIO V, Nelly  and  ALVAREZ F, Pedro. Modelo experimental en el estudio de posibles principios activos antipalúdicos. Rev. perú. med. exp. salud publica [online]. 2006, vol.23, n.3, pp.173-176. ISSN 1726-4634.

Objective: Contribute to the development of an experimental model, which is more economic and of lesser risk, avoiding the use of human sera for continuous culture of Plasmodium falciparum in human erythrocytes for initial studies of medicinal plants with alleged antimalarial possibilities. Materials and methods: The Trager and Jensen technique was followed with minor modifications, such as not using heparinized blood or human sera. The globular package was resuspended in RPMI 1640 in cell culture flasks, supplemented with hepes medium and inactivated bovine fetal serum. The P. falciparum samples in a cryotube was finally harvested. The continuous culture was challenged with watery extract of Bixa orellana (achiote in Spanish). Results: The erythrocyte cultures were achieved without contamination and the spread of P. falciparum was delayed for about 60 days. B. orellana extract caused lysis in the parasited erythrocytes, thus achieving a similar results with the standard Quinine Sulphate. Conclusions: A simple, experimental model was developed for continuous culture of Plasmodium falciparum, low risk in the fact that it does not use human sera, which shows some promise in the study of traditional antimalarial use.

Keywords : Malaria; Plasmodium falciparum; Medicinal plants; Phytotherapy; in vitro.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License