Abstract

PADILLA R, Carlos et al. Expresión y sororreactividad de la lipoproteína recombinante de 43-kda de Bartonella bacilliformis. Rev. perú. med. exp. salud publica [online]. 2006, vol.23, n.3, pp.182-187. ISSN 1726-4634.

Objective: The Bartonella bacilliformis 43-kDa lipoprotein was obtained from its recombinant form (rBbLppB) and then, purified to evaluate sero-reactivity through ELISA. Material and methods: IgG and IgM humanantibodies levels in the sera of patients with confirmed Bartonellosis and sera of patients with other diseases (salmonellosis, Brucellosis and Leptospirosis), when contrasted with rBbLppB were evaluated by ELISA. Sera from some healthy people were used for controls. Results: Sensitivity and specificity of the IgG ELISA were 70,4% and 90% respectively. Also, sensitivity and specificity of the IgM ELISA were 85,2% and 90%, respectively. Conclusions: These results show that ELISA using rBbLppB is highly sensitive and specific and may be considered a good antigen for the diagnosis of Bartonellosis caused by B. bacilliformis.

Keywords : Bartonella bacilliformis; Diagnosis; Enzime-Lynked Immunosorbent Assay; Recombinant proteins; Carrion’s diseases.

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