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Revista Peruana de Medicina Experimental y Salud Publica

Print version ISSN 1726-4634


QUISPE M, Angel et al. Selective in vitro cytotoxic effect of muricin H (Annona muricata acetogenenin) in lung cancer cell cultures. Rev. perú. med. exp. salud publica [online]. 2006, vol.23, n.4, pp.265-269. ISSN 1726-4634.

Objectives: To determine the selective cytotoxic activity of muricin H on a H460 cell line (non-small cell lung cancer). Materials and methods: H460 and 3T3 cell lines (normal murine fibroblasts) were exposed to six concentrations of muricin H (62,5; 15,6; 3,9; 0,98; 0,24; 0,06 µg/mL) and to equal concentrations of 5-fluorouracil (5-FU) used as a positive control. Growth percentages at 48 hours were measured, and then the half-maximal inhibitory concentration (IC50) was determined using linear regression analysis, and Pearson correlation coefficients were calculated. Finally, the selectivity index for each sample was calculated. Results: IC50 values for muricin H, expressed in µg/mL were as follows: <0,06 (r= -0,96; p<0,05) for the H5460 cell line, and 6,16 (r= -0,96; p<0,025) for the 3T3 cell line. IC50 with 5-FU were 0,46 (r= 0,95; p<0,005) for H460, and 0,29 (r= -0,88 P= 0,01) for 3T3. Selectivity indexes for muricin H and 5-FU were <102,6 and 0,63, respectively. Conclusions: In vitro selective cytotoxic activity of muricin H was proven, since it had a greater cytotoxic effect for H460 cell line, and lesser effect upon the 3T3 cell line when compared to 5-FU.

Keywords : Medicinal plants; Phytotherapy; Cytotoxicity; Antineoplastic agents; Cell culture; Lung cancer.

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