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Revista Peruana de Medicina Experimental y Salud Publica

Print version ISSN 1726-4634


ANCCA, Jenny et al. Morphometric, genetic, FEEDING and vectorial characteristics of Panstrongylus herreri from Jaén (Cajamarca) and Cajaruro (Amazonas), Perú. Rev. perú. med. exp. salud publica [online]. 2008, vol.25, n.1, pp.17-25. ISSN 1726-4634.

Objective. To compare the morphology, feeding and the genetic profile of specimens of Panstrongylus herreri from districts of Jaen (Cajamarca) and Cajaruro (Amazonas). Material and methods. Adult specimens of P. herreri were collected, 42 from Jaen and 68 from Cajaruro. Geometric morphometrics was used to evaluate the sexual differences in size and form. The genetic profile was done using the PCR amplification, second internal transcribed spacer (ITS-2) of the ribosomal ADN. The molecular characterization of the isolated trypanosomes was made by the amplification the parasite. We used the precipitin test for identification the feeding sources and thus be able to associate them with the potential reservoir of the parasite. Results. The morphometric analyses showed no difference (p<0,05) in sex between the insects from both departments, but there was difference in the form between females. The genetic profile showed by ITS-2 similar bands of 960 pb in both groups (five insects) from Jaen and Cajaruro. The trypanosoma isolated from P. herreri (only in Cajaruro) was identify as Trypanosoma cruzi TCI (350 pb). Guinea pig blood was the main source of food for the insects. Conclusions. P. herreri from both localities no showed important differences under geometric morphometry and genetic studies. These results indicate the same population with similar origin. The guinea pig blood was the main source of food of P. herrei, may be the principal reservoir for the Chagas disease from this Peruvian area.

Keywords : Panstrongylus; Triatominae; Trypanosoma cruzi; Genetics; Peru.

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