Revista Peruana de Medicina Experimental y Salud Publica
ISSN 1726-4634 versión impresa
PRIETO, Zulita, LEON-INCIO, Julio, QUIJANO-JARA, Carlos et al. Efecto Genotóxico del Dicromato de Potasio EnEritrocitos de sangre periférica de OreochromisNiloticus (Tilapia). Rev. perú. med. exp. salud publica, ene./mar. 2008, vol.25, no.1, p.51-58. ISSN 1726-4634.
ABSTRACT Due multiple reports of the genotoxic and carcinogenic effect of chromium VI and the permanent exposure of the human beings to this element. Objective. Contributing new evidence of the genotoxicity of potassium dichromate using the biological system Oreochromis niloticus "tilapia" through the micronucleus test and the nuclear quantification of buds in erythrocytes of peripheral blood. Material and methods. The individuals were exposed to increasing concentrations (0.0, 0.2, 0.4 and 0.8 ppm) of potassium dichromate. Peripheral blood samples of the branchial arc of each individual were taken at 3th and 7th day of treatment which were processed and colored with Giemsa 5%, erythrocytes in peripheral blood with micronuclei and nuclear buds were quantified. Results. A significant increase of frequencies of micronucleus and nuclear buds in the exposed individuals were registered which were directly proportional to the potassium dichromate concentration (p<0.05). Conclusions. Potassium dichromate caused genetic damage in the cells of O. niloticus.
Palabras llave: Genotoxicity; Chromium; Potassium dichromate; Biological models; Micronucleus test.
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