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Revista Peruana de Medicina Experimental y Salud Publica

Print version ISSN 1726-4634


ANAYA, Elizabeth; MENDOZA, Giovanna; GARCIA-USCAMAYTA, Lourdes  and  FERNADEZ, Ysabel. Indirect elisa of Bartonella bacilliformis total lys ate for quick diagnosis of Carrion disease . Rev. perú. med. exp. salud publica [online]. 2008, vol.25, n.2, pp.243-246. ISSN 1726-4634.

Was developed and standardized an indirect ELISA test with the total lysate of Bartonella bacilliformis from an ATCC strain and a clinical isolation. We selected 86 sera from patients with confirmed diagnosis of Carrion disease by smear and culture, 51 sera of negative patients for non-endemic areas and 32 sera from patients with confirmed serological diagnosis for other bacterial diseases such as brucellosis, leptospirosis, syphilis and Rickettsiosis. We found a sensitivity of 68.6% (95%CI: 58.2-79.0%), specificity of 94.1% (86.7-100%), positive predictive value of 95.2% (89.0 -100%) and negative predictive value of 64.0% (54.3-71.2%), with a cross-reaction with other bacterial aetiologies of 78.1% (25/32). This is not a recommended test to be used as a diagnostic tool for Carrion disease, is continuing studies toward finding a quick test with greater sensitivity.

Keywords : Bartonella infections; Bartonella bacilliformis; Immunologic test; Peru.

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