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Revista Peruana de Medicina Experimental y Salud Publica

versión impresa ISSN 1726-4634

Resumen

MIRANDA, Eduardo; VELARDE, franko; SOMOCURCIO, José  y  AYALA, Eduardo. Evaluation of two immunoblot tests with goat and sheep hydatid antigen for human echinococcosis diagnosis. Rev. perú. med. exp. salud publica [online]. 2010, vol.27, n.2, pp. 209-214. ISSN 1726-4634.

To estimate the diagnosis value of goat and ovine antigen for echinococcosis immunoblot test, 135 serums were used, of which 70 were coming from patients with hydatid disease confirmed by the finding of proto scolex and membrane in the pathology study of surgical piece, 45 from patients with other parasitic diseases and 20 apparently healthy people. The sensitivity, the specificity, positive and negative predictive value of immunoblot test, with hidatyd antigen of goat was of 92.8%, 100%, 100%, 92.8%, respectively, than for ovine antigen was 91.4%, 95.3%, 95.5%, 91.1%, respectively. Kappa index was 0.93 for goat antigen and 0.86 with sheep in relation to the pathological study. We recommended the use of both antigens for the serologic diagnosis of human echinococcosis.

Palabras llave : Echinococcosis; Blotting, western; Sensitivity and specificity; Cross-priming.

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