SciELO - Scientific Electronic Library Online

 
vol.29 número1Efecto del aceite esencial de Aloysia triphylla britton (cedrón) sobre el Trypanosoma cruzi en ratonesEvaluación de la calidad de información sobre el embarazo en páginas web según las guías peruanas índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Artigo

Indicadores

  • Não possue artigos citadosCitado por SciELO

Links relacionados

  • Não possue artigos similaresSimilares em SciELO

Bookmark


Revista Peruana de Medicina Experimental y Salud Publica

versão impressa ISSN 1726-4634

Resumo

MENDOZA, Julio C et al. Experimental efficacy of IgY antibodies produced in eggs against the venom of the peruvian snake Bothrops atrox. Rev. perú. med. exp. salud publica [online]. 2012, vol.29, n.1, pp. 69-75. ISSN 1726-4634.

Objectives. To develop an immunization protocol in order to produce avian IgY immunoglobulins against Bothrops atrox Peruvian snake venom and to evaluate its neutralizing capacity. Materials and methods. Six Hy Line Brown hens were immunized each two weeks using 500μg/doses of B. atrox venom in a period of two months. Each week, eggs were collected for IgY isolation from yolk using two consecutive steps with caprilic acid and ammonium sulfate. Detection of IgY anti-B. atrox were performed by double immunodiffusion, whereas title and cross-reactivity were analyzed using ELISA and Western Blot technics, respectively. Furthermore, letal dose (DL50) and Medium Effective Dose (DE50) were obtained by Probit analysis. Results. As a result of this protocol, chicken IgY’s were obtained in a concentration of 8,5 ± 1,35 mg/yolk mL. DE50 from avian antivenom was 575 μL/venom mg. Cross-reactivity studies showed Bothrops atrox venom share more commom epitopes with Bothrops brazili (47%) than others Bothrops venoms showing Lachesis muta (19%) and Crotalus durissus (12%) venoms a low crossing reactivity, instead. Conclusions. Using this procedure, we could purify chicken IgY with a neutralizant capacity of B. atrox venom which is comparable to the antivenom of equine origin and demonstrate its capacity as a immunoanalitical tool to evaluate the cross reactivity with others peruvian snakes

Palavras-chave : Snake venoms; Antivenins; Neutralization; Bothrops atrox.

        · resumo em Espanhol     · texto em Espanhol     · pdf em Espanhol