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Revista Peruana de Medicina Experimental y Salud Publica
Print version ISSN 1726-4634
Abstract
MORENO-EXEBIO, Luis and GRANDE-ORTIZ, Miguel. Validation of a liquid chromatography method for rifampicin determination in human plasma. Rev. perú. med. exp. salud publica [online]. 2014, vol.31, n.1, pp.56-61. ISSN 1726-4634.
Objectives: To validate the high-performance liquid chromatography method (HPLC) for rifampicin (RFP) determination in human plasma. Materials and methods. A HPLC method for RFP determination in plasma was developed. The separation was performed by reversed-phase chromatography with C18 column and a mobile phase composed of a mixture of acetonitrile and monobasic potassium phosphate buffer solution 0.05 M (38:62 v/v) at 335 nm in which standard rifampicin quinone (RFP-QN) was used. Results. The retention times of RFP and RFP-QN were 7.81 and 12.26 minutes, respectively. The trial was linear from 0.5 to 250 ug/mL. The evaluated parameters of precision, accuracy, selectivity, linearity, and recovery complied with the established international standards for validation of bioanalytical methods. Conclusions. The developed HPLC method is simple, specific, sensitive, selective and linear for a wide range of RFP concentrations in plasma
Keywords : Chromatography, high pressure liquid; Validation studies; Rifampin.