Resumo

ANTITUPA, Isidro et al. Purification of antigenic fraction 27-28 KDa from the metabolic antigen from metabolic secreted-excreted from Fasciola hepatica. Rev. perú. med. exp. salud publica [online]. 2014, vol.31, n.2, pp.288-291. ISSN 1726-4634.

Antigenic fractions of 27-28 kDa from Fasciola hepatica were purified by size-exclusion chromatography for use in the diagnosis of human fasciolosis. Excretion and secretion antigens were obtained from living adult flukes collected from sheep and cattle liver, and cultured in minimum essential medium. The reactivity of the purified antigen and efficacy were assessed by immunoblot test using four sera with human fascioliasis; four sera with other parasites, and two negative sera. We conclude that the purified antigenic fractions do not cross-react with other parasites by immunoblot. Therefore, purified proteins are considered as potential candidates to be used for the diagnosis of human fascioliasis

Palavras-chave : Fasciola hepatica; Purification; Chromatography.

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