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Revista Peruana de Medicina Experimental y Salud Publica
versión impresa ISSN 1726-4634
Resumen
MIRANDA-ULLOA, Eduardo; SANDOVAL-AHUMADA, Roxana; AYALA, Eduardo y VASQUEZ-AMPUERO, Juan. Assessment of dot blot tests and latex agglutination for cysticercosis diagnostic in Peru. Rev. perú. med. exp. salud publica [online]. 2014, vol.31, n.2, pp.297-301. ISSN 1726-4634.
In order to evaluate dot blot tests and latex agglutination for the detection of human cysticercosis with liquid antigen of Taenia solium cysticerci, 125 human sera were used, of which 60 were from people with cysticercosis confirmed by Western Blot, 45 with other parasitic diseases and 20 apparently healthy. The optimal concentration of antigen to impregnate dot blot strips was 0.01 ug/uL, and to impregnate the latex particles was 0.092 ug/uL. For the dot blot test, a sensitivity of 100% and specificity of 87.7% was found. For latex agglutination, a sensitivity of 93.3% and specificity of 89.2% was found. Both tests may be useful and feasible to implement alternatives of serological diagnosis in laboratories in endemic areas of Peru
Palabras clave : Cysticercosis; Enzy me-linked immunosorbent assay; Latex fixation tests; Sensitivity and specificity.