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Revista Peruana de Medicina Experimental y Salud Publica

Print version ISSN 1726-4634


RIVERA-JACINTO, Marco et al. TEM and CTX-M extended-spectrum beta-lactamase in Klebsiella spp and Escherichia coli isolates from inanimate surfaces of hospital environments. Rev. perú. med. exp. salud publica [online]. 2015, vol.32, n.4, pp.752-755. ISSN 1726-4634.

The aim of the study was to determine the genotype of 15 ESBL strains of Enterobacteriaceae resistant to beta-lactams, isolated from inanimate surfaces and phenotypically characterized as producing extended-spectrum beta-lactamase. After evaluation and screening of the bacterial strains, a PCR was conducted to amplify fragments of 1078 bp and 544 bp corresponding to type TEM and CTX-M ESBL. Eleven strains presented both fragments at the time and only three had blaCTX-M. In conclusion, the presence of ESBL genes in cultures from the environment was demonstrated, some of which may belong to more than one type. This information could serve as a basis for implementing preventive measures to prevent the transmission of multiresistant bacteria from inanimate surfaces to patients, mainly in critical hospital areas

Keywords : beta-Lactamases; Cross infection; Klebsiella pneumoniae; Escherichia coli; Polymerase chain reaction.

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