Abstract

ROJAS JAIMES, Jesús; GIRALDO-CHAVEZ, Jorge; HUYHUA-FLORES, Yudit  and  CACERES-NAKICHE, Tatiana. Mycobacteria identification in solid culture by inverted phase microscopy and Ziehl-Neelsen Stain. Rev. perú. med. exp. salud publica [online]. 2018, vol.35, n.2, pp.279-284. ISSN 1726-4634.  http://dx.doi.org/10.17843/rpmesp.2018.352.3471.

With the aim of identifying morphological differences in colonies of different species of mycobacteria by means of inverted phase microscopy, nine species of high-prevalence pathogenic mycobacteria were seeded in 7H11 solid medium and were observed for up to 21 days. Additionally, Ziehl-Neelsen (ZN) stains were carried out for each one of them. No morphological variations were identified among the species grown on solid medium; however, it was identified that all are able to form cords in solid medium in an early stage of growth, being corroborated by the Ziehl-Neelsen stain in Mycobacterium tuberculosis. so techniques based on detecting M. tuberculosis depending on the identification of the cords should be taken with caution so as not to generate a bad diagnosis, so microbiological characteristics could also be established as a minimum time to detect growth, temperature of growth and bacillary characteristics based on the Ziehl-Neelsen stain for each species studied.

Keywords : Tuberculosis; Mycobacterium tuberculosis; Mycobacterias; Culture media.

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