SciELO - Scientific Electronic Library Online

 
vol.35 issue4Bibliometric indicators of papers published in Revista Peruana de Medicina Experimental y Salud Publica, 2010-2017Characterization of metallo-β-lactamase in clinical isolates of Pseudomonas aeruginosa retrieved from patients hospitalized in the Central Military Hospital author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO

Share


Revista Peruana de Medicina Experimental y Salud Publica

Print version ISSN 1726-4634

Abstract

PALACIOS-SALVATIERRA, Rosa et al. Rickettsial species in human cases with non-specific acute febrile syndrome in Peru. Rev. perú. med. exp. salud publica [online]. 2018, vol.35, n.4, pp.630-635. ISSN 1726-4634.  http://dx.doi.org/10.17843/rpmesp.2018.354.3646.

With the objective of molecularly characterizing rickettsial isolates from humans with non-specific acute febrile syndrome, a cross-sectional descriptive study was conducted, with isolates propagated in Vero ATCC cellular cultures and alternative lines, verifying the viability by means of Indirect Immunofluorescence. Prior to DNA extraction, the gltA gene was amplified by means of conventional PCR, and its sequence was analyzed. Twelve isolates were amplified, five with sufficient DNA so as to sequence them, exhibiting compatibility with R. asembonensis in four, and a close identity with Coxiella burnetti in one. At least three of seven alternative cellular lines showed significant yield in sub-cultures. R. asembonensis was identified in four isolates of humans with non-specific acute febrile syndrome, coming from the regions of Ayacucho, Cajamarca, and Madre de Dios in Peru, and Coxiella burnetti in one coming from the Loreto region.

Keywords : Rickettsia; Polymerase Chain Reaction; Peru.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License