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Revista Peruana de Medicina Experimental y Salud Publica
Print version ISSN 1726-4634
Abstract
MANTARI TORPOCO, Carina Rosario; BERROCAL HUALLPA, Alfredo Miguel; ESPINOZA-CULUPU, Abraham Omar and LOPEZ-INGUNZA, Ricardo Luis. Molecular characterization of the rabies virus nucleoprotein in dogs from Arequipa, Peru. Rev. perú. med. exp. salud publica [online]. 2019, vol.36, n.1, pp.46-53. ISSN 1726-4634. http://dx.doi.org/10.17843/rpmesp.2019.361.3938.
Objective. To characterize the nucleoprotein (N) and establish the origin of the rabies virus in dogs coming from Arequipa. Materials and Methods. Thirty samples of nervous tissue from the departments of Arequipa and Puno were analyzed. Total RNA was extracted from the samples and cDNA was synthesized to amplify the nucleoprotein gene, sequence it, and perform bioinformatics analysis. Results. A defined group was formed with respect to the external group (European bat lyssavirus). This group was classified into two subgroups, one constituted by samples coming from Puno and Arequipa (subgroup A), and another one by samples from Puno (subgroup B), exhibiting a nucleotide identity of 99.9% in subgroup A. This group was classified in two subgroups, one constituted by samples coming from Puno and Arequipa (subgroup A), and another one by samples from Puno (subgroup B), observing a nucleotide identity of 99.9% in subgroup A. Conclusions. The groupings of viral sequences show that the cases of canine rabies reported in Arequipa are the result of the expansion of canine rabies from the endemic region of Puno.
Keywords : RNA Virus; rabies virus; high-performance nucleotide sequencing; dogs; Peru.