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Revista Peruana de Medicina Experimental y Salud Publica

Print version ISSN 1726-4634


BASTIDAS, Betsi; MENDEZ, María V; VASQUEZ, Ysvette  and  REQUENA, Dayana. Typification of the staphylococcal chromosome cassette of methicillin-resistant Staphylococcus aureus in the state of Aragua, Venezuela. Rev. perú. med. exp. salud publica [online]. 2020, vol.37, n.2, pp.239-245. ISSN 1726-4634.


Typify the SCCmec cassette in methicillin-resistant strains of Staphylococcus aureus in clinical isolates from health centers in the State of Aragua-Venezuela and compare the presence of SCCmec genotypes among the state health centers and according to the type of infection.

Materials and methods:

81 MRSA strains from four health centers of the Aragua-Venezuela State were studied. Methicillin resistance was performed with the Kirby-Bauer method with oxacillin (1 µg) and cefoxitin (30 µg) disks. The mecA gene and SCCmec were analyzed by the multiple PCR technique.


Only 55 isolates (67.9%) amplified the mecA gene, and 24 strains (43.6%) amplified SCCmec. SCCmec type I was the most frequency, followed by SCCmec IV and SCCmec III, representing 62.5%, 25% and 12.5%, respectively. SCCmec I was predominant in health center A (80%), while in B and C 60% and 100% respectively were SCCmec IV. At health center D, 50% turned out to be SCCmec I and 50% SCCmec IVd. A relationship was found between the SCCmec and the health center with statistical significance. SCCmec I predominated in skin and soft tissue and respiratory infections with 63.2% and 50%, respectively. There was no association between genotype and type of infection with a p value greater than 0.05.


The prevalence of SCCmec I and IV will allow establishing new measures in the use of antibiotics and epidemiological control.

Keywords : Staphylococcus aureus; SCCmec; mecA gene; Molecular Epidemiology; Staphylococcus aureus methicilin resistent.

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