Abstract

SALINAS-SALCEDO, Iván Alexander; SALDANA-JIMENEZ, José Antonio; GUEVARA-CANALES, José Manuel  and  GONZALES-ESCALANTE, Edgar. Utility of flow citometry for detecting metallo-beta-lactamase-producing Pseudomonas aeruginosa. Rev. perú. med. exp. salud publica [online]. 2020, vol.37, n.4, pp.700-704.  Epub Nov 11, 2020. ISSN 1726-4634.  http://dx.doi.org/10.17843/rpmesp.2020.374.4825.

In order to determine the utility of flow cytometry for detecting metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa, we used genotypically characterized P. aeruginosa isolates from the Molecular Epidemiology and Genetics Laboratory of the Universidad Nacional Mayor de San Marcos. A total of 29 isolates (17 MBL-producing and 12 non-MBL-producing) were analyzed with the FACSCalibur (Becton Dickinson) cell viability kit. Two treatments were used, one with meropenem and the other with meropenem-EDTA. A significant difference between MBL and non-MBL-producing P. aeruginosa was demonstrated using the fluorescence ratio in non-living cells, considering a cut-off point of >1.6. We determined a sensitivity of 94.1% and a specificity of 100%. Flow cytometry represents an alternative for the detection of MBL-producing P. aeruginosa.

Keywords : Pseudomonas aeruginosa; Carbapenems; beta-Lactamases; beta-Lactam resistance, Flow Cytometry.

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