Abstract

ZARATE, Marjhory et al. Frequency of colistin resistance in Pseudomonas aeruginosa: first report from Peru. Rev. perú. med. exp. salud publica [online]. 2021, vol.38, n.2, pp.308-312.  Epub May 26, 2021. ISSN 1726-4634.  http://dx.doi.org/10.17843/rpmesp.2021.382.6977.

This study aimed to determine the frequency of colistin resistance in Pseudomonas aeruginosa isolates obtained from three healthcare facilities in Lima and cryopreserved at the Laboratorio de Resistencia Antimicrobianos e Inmunopatología of the Universidad Peruana Cayetano Heredia (UPCH). The colistin broth disk elution method was used for the phenotypic identification of colistin resistance. We detected the expression of the mcr-1 gene by using the phenotypic diffusion method with combined colistin and ethylenediaminetetraacetic acid (EDTA) disks; and polymerase chain reaction (PCR) was used for molecular identification of the gene. Of the 97 isolates, 7 (7.2%) were resistant to colistin; however, none carried the mcr-1 gene. This is the first report from Peru on clinical isolates of colistin-resistant Pseudomonas aeruginosa, which suggests the need for implementation of appropriate methodologies for the epidemiological surveillance of colistin-resistant pathogens.

Keywords : Pseudomonas aeruginosa; Antimicrobial Drug Resistance; Colistin; Peru.

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