SciELO - Scientific Electronic Library Online

 
vol.38 issue4Trends in drugs usage for COVID-19 during the first wave of the pandemic in a hospital in Lima, PeruOcular manifestations of toxocariasis in schoolchildren from the state from the Anzoátegui state in Venezuela author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO

Share


Revista Peruana de Medicina Experimental y Salud Publica

Print version ISSN 1726-4634

Abstract

PULIDO-COLINA, Angie; PASTRANA, Javier Soto; VALENCIA-BAZALAR, Esther  and  APESTEGUI, Milagros Zavaleta. Molecular characterization of virulence (lmb, bca and rib) and macrolid resistance genes (ermB, ermTR and mefA) in clinical isolates of Streptococcus agalactiae. Rev. perú. med. exp. salud publica [online]. 2021, vol.38, n.4, pp.615-620.  Epub Dec 13, 2021. ISSN 1726-4634.  http://dx.doi.org/10.17843/rpmesp.2021.384.8726.

The aim of the study was to molecularly identify virulence and macrolide resistance genes in clinical isolates of Streptococcus agalactiae (GBS), recovered in 2019 from vaginal discharge (n=9) and urine (n=22), from two health facilities in Lima. Identification and antimicrobial susceptibility were determined by the Vitek® 2 automated system, identification was confirmed phenotypically; macrolide resistance was determined by the D-test method. Identification of virulence genes (lmb, bca and rib) and macrolide resistance genes (ermB, ermTR and mefA) was carried out by polymerase chain reaction (PCR). The predominant macrolide resistance phenotype and genotype were cMLSb (12/31) and ermB (11/31); the most frequent virulence gene was lmb (23/31). All were sensitive to penicillin, ampicillin and vancomycin. These findings show the need to implement molecular epidemiology studies that allow adequate knowledge and follow-up of GBS in Peru.

Keywords : Streptococcus agalactiae; PCR; Genes; Virulence; Drug Resistance; Macrolides; Microbial Sensitivity Test.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )