Abstract

HIDALGO, Manuel et al. Evaluation of in vitro susceptibility to sparteine in four strains of Mycobacterium tuberculosis. Rev. perú. med. exp. salud publica [online]. 2022, vol.39, n.1, pp.77-82.  Epub Mar 31, 2022. ISSN 1726-4634.  http://dx.doi.org/10.17843/rpmesp.2022.391.10136.

Sparteine is an alkaloid with bacteriostatic activity on the genus Mycobacterium. The aim of this study was to evaluate the antimicrobial activity of sparteine on the growth of 4 ATCC strains of Mycobacterium tuberculosis (susceptible, resistant to isoniazid, resistant to rifampicin and multidrug-resistant) in vitro. Validation of bactericidal activity of sparteine sulfate was carried out through an adaptation of the Microscopic-Observation Drug-Susceptibility (MODS) method according to the guidelines of the Peruvian National Health Institute. The results demonstrate that at concentrations of 25; 50 and 100 Mm of sparteine sulfate, there is no development of colony-forming units in any of the 4 evaluated strains. Our results demonstrate the potential in vitro antimicrobial effect of sparteine on multidrug-resistant tuberculosis.

Keywords : Mycobacterium tuberculosis; Tuberculosis; Resistant Tuberculosis; Sparteine; Sparteine Sulfate; Alkaloid.

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