Abstract

ZAPANA, Enrique Mamani-; VILCAPOMA-DIAZ, Meyling  and  ORTIZ-MUCHOTRIGO, Nazario. Detection of human papilloma virus in breast cancer biopsies using PCR and immunohistochemistry at the santa rosa hospital during 2019. Rev. perú. med. exp. salud publica [online]. 2022, vol.39, n.4, pp.450-455.  Epub Dec 15, 2022. ISSN 1726-4634.  http://dx.doi.org/10.17843/rpmesp.2022.394.11069.

The aim of this study was to determine the presence of Human Papillomavirus (HPV) type 16 and 18 in biopsies of paraffin-embedded breast tissue from patients with clinically diagnosed breast cancer. 32 paraffin-embedded breast cancer biopsies were analyzed in order to detect HPV DNA by real-time PCR, the primers were directed at the E6 gene. The histological type, histological grade and overexpression of C-erB2 and Ki-67 were evaluated by immunohistochemistry. 84.38% (27) of the samples were positive for HPV, 25% (8) were positive for HPV-16 and 59.38% (19) were positive for HPV-18. Mixed infection was found in 15.63% (5) of the samples. Overexpression of C-erbB2 and Ki-67 was seen in 6.25% (2) of the samples positive for HPV-16 and in 15.63% (5) samples positive for HPV-18. HPV-16 and HPV-18 DNA was detected in the biopsy samples analyzed by real-time PCR.

Keywords : Human Papillomavirus type 16 and 18; breast cancer; biopsy; qPCR; IHC.

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