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Horizonte Médico (Lima)

Print version ISSN 1727-558X

Abstract

MOYA-SALAZAR, Jeel; PIO-DAVILA, Liz; TERAN-VASQUEZ, Alfonso  and  OLIVO-LOPEZ, José. Diagnosis yield of blood agar with filter against karmali agar for isolation of Campylobacter in stool culture. Horiz. Med. [online]. 2016, vol.16, n.3, pp.58-65. ISSN 1727-558X.

Objective: To evaluate the diagnostic yield of blood agar with filter (BAF) compared to Karmali agar (KA) for the isolation of Campylobacter spp in stool culture. Material and methods: A prospective cross section experimental investigation was performed at "San Bartolome" Mother- Child Teaching National Hospital. Stool samples were evaluated with a reactive functional stool test for inflammation. Samples were inoculated in BAF and KA and incubated for up to 72 hours in microaerophilic conditions. Sample differentiation was performed by the Hippurate hydrolysis test. Results: A total of 287 positive samples were collected. The isolation of Campylobacter in BAF and KA were of the order of 78.3% and 21.7%, respectively. The sensitivity was 90.9% for BAF. The average growth time was 32.7 ± 11 hours (p <0.59) and the contamination of culture media from positive patients was of the order of 61.7% for KA. 74.8% of the isolated species were Campylobacter non- jejuni. Conclusions: BAF has a higher diagnostic yield than KA for the isolation of Campylobacter in a relatively shorter time providing to be more cost-effective than selective isolation. In addition, Campylobacter non-jejuni was mainly isolated in neonates and infants, where most infections occur

Keywords : campylobacter; agar; blood; diarrhea; karmali agar; diagnosis.

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