Abstract

WALDIR, Estela et al. Production of lactic acid by Lactobacillus plantarum L10 on batch and continuous cultivation   . Rev. peru biol. [online]. 2007, vol.14, n.2, pp.271-276. ISSN 1727-9933.

Strain of Lactobacillus plantarum L10 was tested at laboratory scale, in order to evaluate the production of lactic acid on batch and continuous cultivation. Furthermore, optimization of culture medium and cultivation conditions were carried out too. The best parameters obtained for lactic acid production on batch cultivation were: Y_P/S 86,1%; P_P 5,4 g/L/h; joined to Y_X/S 13,2%; P_X 1,2 g/L/h and n = 0,2 h^-1, the medium composition was as follows: glucose 70 g/L; yeast extract 12,1 g/L; KH₂PO₄ 1,2 g/L; (NH₄)₂HPO₄ 1,2 g/L; ammonium citrate 3,0 g/L; MgSO₄. 7H₂O 0,3 g/L and MnSO₄. 4H₂O 0,03 g/L. Respecting to continuous cultivation the best parameters obtained for production of lactic acid were: Y_P/S 96%; P´_P 6,0 g/L/h; joined to Y_X/S 19%; P´_X 1,2 g/L/h; and dilution rate (D) 0,46 h^-1.

Keywords : Lactic bacteria; Lactobacillus plantarum; batch cultivation; continuous cultivation; chemostat; lactic acid..

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