Abstract

BORGES GARCIA, Misterbino et al. In vitro conservation of Dioscorea alata L. clon caraqueño (Dioscoreaceae). Rev. peru biol. [online]. 2009, vol.16, n.2, pp.203-208. ISSN 1727-9933.

In this study, we report an efficient method for conservation in vitro of Dioscorea alata L. clone caraqueño during 9 and 12 months based on the culture medium modification (MS to 75% + vitamins MS + sucrose 30 g.L^-1 + activated charcoal 2 g.L^-1) with different manitol (0; 0,5 and 1,5%) and BAP (0; 0,1 mg.L^-1) levels. At 9 and 12 months of in vitro conservation, the following evaluations were determined: survival (%), leaf senility (%), shoot length, buds and microtuber explant count. Plant regeneration (%), shoot length, leaf number and bud explant count were determined at 8 weeks of the nodal cutting regeneration (from vitroplants conserved for 9 and 12 months) in the culture medium (MS to 75% + vitamins MS + sucrose 30 g.L^-1 + activated charcoal 2 g.L^-1). .Shoot length, leaf number and bud explant count were evaluated at 5 weeks during the conventional micropropagation. The cultivation variants formed by media MS to 75% + vitamins MS + sucrose 30 g.L^-1 + activated charcoal 2 g.L^-1 and MS to 75% + vitamins MS + sucrose 30 g. l^-1 + activated charcoal 2 g.L^-1 + BAP 0, 1 mg.L^-1 allowed an effective way the conservation of in vitro plants from nodal cutting of clone caraqueño yam during 9 and 12 months with high survival percentages, a significant number of microtubers, smallest leaf senility percentages and 100% regeneration in whole plants with a normal growth in micropropagation conditions.

Keywords : yam; medium-term conservation; cultivation in vitro; mannitol; BAP.

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