Abstract

RODRIGUEZ, Ángel et al. Differentiation morphological and by Inter simple sequence repeats (ISSR) of species of genus Plukenetia (Euphorbiaceae) from Peruvian Amazon: suggestion for a new species. Rev. peru biol. [online]. 2010, vol.17, n.3, pp.325-330. ISSN 1727-9933.

In this work, five taxa of the genus Plukenetia from Peruvian Amazon: P. brachybotrya, P. loretensis, P. polyadenia, P. huayllabambana, P. volubilis (from San Martin) and one morphotype (P. volubilis, from Cusco) were studied. The 126 specimens used were identified by morphological keys (shapes of leaves, stems and seed; basilaminar glands position) and by ISSR molecular markers (CAA, CAG, GACA). The morphological analysis of the different taxa studied allowed us to clearly identify five described species: P. brachybotrya, P. loretensis, P. polyadenia, P. volubilis (from San Martin) and Plukenetia huayllabambana. Both morphotypes of P. volubilis (San Martin and Cusco) were distinguished for the position of the glands, seed size and shape of the stem. The results provided by Correspondence Factorial Analysis (AFC) and supported by the fixation index (Fst), genetic distance and the dendogram estimated by the UPGMA method, show a strong differentiation among the six taxa, corroborating the molecular taxonomic identity of the five species morphologically described. Also, results (Fst and Genetic distance) suggest that P. volubilis (from Cusco) could be a new Sacha Inchi species, not yet described to science.

Keywords : Sacha inchi; ISSR; Plukenetia; Biodiversity; Amazon.

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