Abstract

CARHUARICRA E, Karina; OLIVERA S, Julio; GONZALES A, Javier  and  RODRIGUEZ L, Juan. Introduction and multiplication in vitro of garlic culture var. Morado Barranquino. Rev. peru biol. [online]. 2012, vol.19, n.3, pp.341-344. ISSN 1727-9933.

We determine the effect of modified culture medium using different growth regulators concentrations (auxins / cytokinins) for regeneration and propagation of in vitro garlic plants (Allium sativum L.) var. ‘Morado Barrranquino’. In the introduction stage meristem tips were cultured in test tubes up to regeneration. It was determined that medium MSM + IBA 0.6 mg/L was most suitable for the meristem tip differentiation and development. 13 weeks old microplants obtained on introduction stage were used for multiplication, obtaining the formation of a cluster after each subcultive. The best multiplication ratio was obtained in MMA medium + IAA 2.5 mg/L + KIN 5.0 mg/L and MSM medium + 2iP 1.5 mg/L with 4.71 and 4.79 respectively

Keywords : Allium sativum; explants; in vitro; meristem caulinar; subcultive.

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