Abstract

OLIVERA-GONZALES, Percy; ESPINOZA DEL RIO, Rosa  and  TAMARIZ-ANGELES, Carmen. In vitro multiplication and somatic embryogenesis of Perezia pinnatifida (Asteraceae) medicinal Andean plant. Rev. peru biol. [online]. 2017, vol.24, n.3, pp.323-328. ISSN 1727-9933.  http://dx.doi.org/10.15381/rpb.v24i3.13911.

This work inform on in vitro propagation of the "valeriana" Perezia pinnatifida (Humb. & Bonpl.) Wedd. Shoot multiplication and indirect somatic embryogenesis methodologies were performed. The basal culture medium for all stages was Murashige and Skoog, middle of salts supplemented with 2.0% sucrose, 0.3% phytagel and pH 5.67; the treatments were prepared with or without phytohormones. The hormonal supplements for the shoot multiplication were: BAP 1.0 mg.L-1 + ANA 0.01 mg.L-1, and BAP 1.0 mg.L-1; for embryogenic callus induction were: ANA or 2,4-D (1.0 mg.L-1 and 2.0 mg.L-1); and for the embryo germination were: BAP (0.5 and 1.0 mg.L-1) or BAP 0.5 mg.L-1 + ANA 0.05 mg.L-1. BAP 1.0 mg.L-1 produced the higher number buds. For somatic embryogenesis, ANA 1.0 mg.L-1 induced a greater area of embryogenic callus, and BAP 0.5 mg.L-1 allowed major germination of the somatic embryos

Keywords : Planta medicinal; Micropropagación, embrión somático; Cultivo de tejidos vegetales; propagación clonal; Medicinal plant; Micropropagation; Somatic embryo; Plant tissue culture; clonal propagation.

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