Abstract

RAMOS MARTINEZ, Lissette  and  ROSALES RIMACHE, Jaime. Association between tobacco use and micronuclei count in buccal epithelium cells from students of a private college, Ica, Peru (2017). Acta méd. Peru [online]. 2018, vol.35, n.4, pp.216-222. ISSN 1728-5917.

Objective. To assess the relationship between micronuclei count in buccal epithelium cells and tobacco use in students from a private college in Ica. Materials and Methods. This is a cross-sectional study that was performed in college students. Samples from their buccal epithelium were obtained, and they were stained with Giemsa aiming to perform micronuclei count in 2000 cells per sample, using light microscopy at 100 X. Tobacco use was obtained by self-reporting and using a nominal dichotomic scale (yes/no), and data were collected with respect to frequency and time consuming cigarettes. Results: One hundred and twenty nine students participated, 58.1% were female and their average age was 24.9 ± 5.4 years. One quarter of all participants (25.6%) were active smokers, and their average micronuclei count was 3.9 ± 2.4, while the count in non-smokers was 0.5 ± 1.2. A directly proportional relationship was found between tobacco use and micronuclei count (p<0.001). Age, sex, number of cigarettes per day and time being a smoker did not generate significant differences in micronuclei counts. It was found that students with marked tobacco use had a 9.7 times (95% CI: 4.4-21.6) higher risk for having more than 2 micronuclei/2000 cells, being this adjusted for age and sex. Conclusion. Tobacco use is associated to micronuclei count in buccal epithelial cells from students in a private college in Ica.

Keywords : Micronucleus tests; Tobacco use disorder; Epidemiologic models; Student health services.

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