Abstract

FERNANDEZ JERI, Yadira; ZAVALETA, Amparo I  and  MUNOZ JAUREGUI, Ana María. Optimization of the process of immobilization of lipase produces by Marinobacter sp Ch27 in octadecilo - sepabead. Rev. Soc. Quím. Perú [online]. 2016, vol.82, n.4, pp.480-490. ISSN 1810-634X.

The aim of these research was the optimize in the support hydrophobic octadecilo-sepabead by interfacial adsorption, for which the experimental design was used Box-Behnken 3 factors and 3 levels with a total of 12 experiments and 3 replicas on the central point. The variables and their levels selected were: force Ionic (2.5, 5.0 and 10.0 mM), time (30, 60 and 120 min) and pH (6.0; 7.0 and 8.0) and them variable answers were the activity of the enzyme immobilized (U/g support) and the percentage immobilization. The optimum parameters of the immobilization process were: pH 6.62, 30 min and ionic strength 10 Mm. After adjust the parameters to the model polynomial quadratic, is obtained 11,16 U/g support for the activity of lipase immobilized, with 81.50% and 74.0% of activity relative and performance of immobilization respectively

Keywords : Lipase; Marinobacter sp; inmovilization; octadecyl - sepabead; response surface methodology.

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