Abstract

FLORES RAMOS, Leenin  and  RUIZ SOTO, Anthony. Implementation of analytical methodology for quantification of proteins in Arthrospira platensis microalgae. Rev. Soc. Quím. Perú [online]. 2017, vol.83, n.4, pp.371-381. ISSN 1810-634X.

The Hartree-Lowry method was developed and implemented for quantification of protein in Arthorspira platensis microalgae by spectrophotometry UV-Vis. In order to achieve this goal, the protein extraction factors were optimized by Box Behnken design of response surface and the following results of the implementation parameters were obtained: the linearity showed a coefficient of determination (R2) greater than 0.995, a coefficient of variation less than 2% for repeatability, a percentage recovery within 94% to 101%, the limit of detection and limit of quantification were 2.36 μg/mL and 7.86 μg/mL respectively, and the robustness showed that some parameters such as sample weight and volume of reagent C presented high influence in the evaluated factors.

Keywords : Spectrophotometry; microalgae; Box Behnken; Youden-Steiner.

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