SciELO - Scientific Electronic Library Online

vol.85 issue4Purification and some biochemical and molecular properties of an new not myotoxic phospholipase A2 from Bothrops atrox snake venom author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO


Revista de la Sociedad Química del Perú

Print version ISSN 1810-634X


RODRIGUEZ BEST, Carmen Alicia; CERONI GALLOSO, Mario; VILLEGAS SILVA, Elvito Fabián  and  REBAZA CARDENAS, Teresa Dámaris. Total selenium and celular viability for obtaining selenium-yeast. Rev. Soc. Quím. Perú [online]. 2019, vol.85, n.4, pp.518-526. ISSN 1810-634X.

Selenium-yeast is obtained by the culture of Saccharomyces cerevisiae LALVIN Bourgovin RC 212 in the medium YEPD, with sodium selenite addition (concentrations of 2 to 10 mg Se(IV)/L) during exponential growth phase, after 48 hours at 30°C and 200 rpm. Total intracellular selenium quantification in the selenium-yeast is feasible with the starch-iodine spectrophotometric method at 590 nm and the addition of 8 mg Se(IV)/L yields the most biomass, a >90% cellular viability, pink coloration and a total intracellular selenium of 738±30 mg/kg in dry basis.

Finally, the used methodology to find the cellular viability and the analytical method proposed to determine the total intracellular selenium are accessible for monitoring the bioprocess.

Keywords : total selenium; selenium-yeast; selenite; cellular viability.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )