Resumen

RODRIGUEZ BEST, Carmen Alicia; CERONI GALLOSO, Mario; VILLEGAS SILVA, Elvito Fabián  y  REBAZA CARDENAS, Teresa Dámaris. Total selenium and celular viability for obtaining selenium-yeast. Rev. Soc. Quím. Perú [online]. 2019, vol.85, n.4, pp.518-526. ISSN 1810-634X.  http://dx.doi.org/10.37761/rsqp.v85i4.264.

Selenium-yeast is obtained by the culture of Saccharomyces cerevisiae LALVIN Bourgovin RC 212 in the medium YEPD, with sodium selenite addition (concentrations of 2 to 10 mg Se(IV)/L) during exponential growth phase, after 48 hours at 30°C and 200 rpm. Total intracellular selenium quantification in the selenium-yeast is feasible with the starch-iodine spectrophotometric method at 590 nm and the addition of 8 mg Se(IV)/L yields the most biomass, a >90% cellular viability, pink coloration and a total intracellular selenium of 738±30 mg/kg in dry basis.

Finally, the used methodology to find the cellular viability and the analytical method proposed to determine the total intracellular selenium are accessible for monitoring the bioprocess.

Palabras clave : total selenium; selenium-yeast; selenite; cellular viability.

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