SciELO - Scientific Electronic Library Online

 
vol.6 número4Modelación matemática del efecto de la temperatura en la actividad y la estabilidad térmica de la inulinasa de Kluyveromyces marxianus NRRL Y-7571¿El enfriamiento de los frutos y el recubrimiento de película de las semillas afecta al potencial de germinación de la uchuva? índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

  • Não possue artigos citadosCitado por SciELO

Links relacionados

  • Não possue artigos similaresSimilares em SciELO

Compartilhar


Scientia Agropecuaria

versão impressa ISSN 2077-9917

Resumo

CEDANO ROMERO, José; RODRIGUEZ, Alicia  e  SICHE, Raúl. Maximizing content of Omega-3 (EPA and DHA) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in supercritical CO2 conditions. Scientia Agropecuaria [online]. 2015, vol.6, n.4, pp.313-323. ISSN 2077-9917.  http://dx.doi.org/10.17268/sci.agropecu.2015.04.08.

The aim of this study was to optimize the content of EPA and DHA in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in structured triacylglycerols (TAGs). For this purpose, nonspecific lipase B from Candida antarctica immobilized in a supercritical CO2 was used. Crude salmon oil obtained from the industrial byproducts was used to obtain LCPUFA concentrate. Initially, a LCPUFAs concentrate was obtained by basic hydrolysis and posterior complexation with urea. Subsequently the process variables were optimized enzymatic acidolysis were optimized using a central composite rotational design 25-1 + star, with 5 factors and 30 experimental trials, based on the response surface methodology. The optimal conditions that maximized the content of EPA and DHA to 3.92 g/100 g TFA and 9.09 g/100 g TFA, respectively in the purified TAGs corresponded to a LCPUFA percentage 71.71% and canola oil percentage 28.29%, temperature 57.8 °C, pressure 172.0 bar, time 23.97 h enzyme percentage of 7.74%.

Palavras-chave : Optimization; supercritical carbon dioxide; structured triacylglycerols; docosahexaenoic acid (DHA); eicosapentaenoico acid (EPA).

        · resumo em Espanhol     · texto em Inglês     · Inglês ( pdf )