Abstract

SANCHEZ SUAREZ, Héctor et al. Isolation of bacterial inhibitory peptides from lactic acid bacteria of the piglet digestive tract and identification by proteomic test. Scientia Agropecuaria [online]. 2017, vol.8, n.4, pp.437-443. ISSN 2077-9917.  http://dx.doi.org/10.17268/sci.agropecu.2017.04.15.

In pig feed antibiotics are used which are a latent risk to animal and public health. Microorganisms are acquired from the environment during different stages of life, some bacteria that colonize and become part of the natural digestive flora and exert antibiotic action (extracellular substances called bacteriocins), Extracellular substances of eight lactic acid bacteria native to the digestive tract (Sus scrofa domesticus) were isolated from two post weaned piglets; BALs were identified by biochemical tests and tests of antagonism against Echerichia fergunsonii and Shigella sannei. The molecular identification of the bacteria and the protein substance was performed proteomically mediated by the Q proteome ™ method (method of extraction of the protein directly from the culture of bacteria) where the bacterial digestion was initially performed with the Kit, the products were subjected to Migrations in the polycrylamide gel, selecting those of 5 and 2500 KD cut from the gel and a second digestion with conventional trypsin method. The products were analyzed by mass spectrometry in Maldi TOF TOF, obtaining fragments of peptides that were molecularly compared to organisms belonging to Bacillos and later with genus Lactobacilli, where two peptides named ornithine monooxygenase (Bacillus firmus) and RNase J family Beta-CASP ribonuclease (Lactobacillus saerimneri) peptides that have antimicrobial, inhibitory effect of bacteria that can be bacteriocins

Keywords : Bacteriocin; proteomics; antagonisms; BAL; native bacteria.

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