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Scientia Agropecuaria

Print version ISSN 2077-9917

Abstract

SCOTTO ESPINOZA, Carlos. Report of a second introduction of fluorescent transgenic ornamental fish to Peruvian territory: Skirt tetra fish case (Gymnocorymbus ternetzi; Boulenger, 1895). Scientia Agropecuaria [online]. 2018, vol.9, n.1, pp.153-156. ISSN 2077-9917.  http://dx.doi.org/10.17268/sci.agropecu.2018.01.16.

Where their fluorescence was identified with UV light and the same group of primers used to identify red fluorescence zebrafish (Danio rerio) together with negative controls of the zebrafish without the RFP transgene and the transgenic Medaka (Oryzias latipes) fish were amplified by PCR a GFP transgene that they did not amplify. However, both the white and black skirt tetra fish without the RFP transgene and the fluorescent red transgenic skirt tetra fish with RFP did not amplify any bands. A new variant of the red fluorescent protein gene used to obtain red fluorescent skirt tetra fish other than the one used to generate transgenic zebrafish fluorescens.

Keywords : Gymnocorymbus ternetzi; fluorescence; RFP; transgenic.

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