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Scientia Agropecuaria
versión impresa ISSN 2077-9917
Resumen
ORDINOLA-ZAPATA, Alberto; VIEYRA-PENA, Enedia G; RAMIREZ-SEGURA, Beder E y SAAVEDRA-OLIVOS, Katherine Y. Genetic Diversity of Ucides occidentalis (Ortmann 1897) (Crustacea: Decapoda: Brachiura) based on 16S rRNA gene in Tumbes, Peru. Scientia Agropecuaria [online]. 2018, vol.9, n.2, pp.259-267. ISSN 2077-9917. http://dx.doi.org/10.17268/sci.agropecu.2018.02.11.
The mangrove crab Ucides occidentalis, is a key specie in the mangrove for recycle up to 84% of its leaves, also it is the crab most exploited in the Tumbes region, so much so that its population has dramatically shrunk, to 35.8% in 11 years, this situation can lead to repopulation needs to b e done, which to be done properly requires knowledge of their population genetics. In this study we sought to determine the polymorphism, genetic diversity and population genetic structure of U. occidentalis in the mangroves of Tumbes, based on the study of a 16S rRNA gene fragment. Of 38 U. occidentalis DNA samples collected from the mangrove Tumbes, we amplified and sequenced the 16S rRNA gene fragments, which when analyzed showed polymorphism (polymorphism rate of 52.64%), a high genetic diversity (indicated by the high number of haplotypes: 16 and haplotype diversity: 0.721; and the average of differences in nucleotide: 1.677 and nucleotide diversity: 0.00381), and a low population genetic structure (assessed by MANOVA with genetic variability among populations of 11%). Similarly, it became clear that there was no relationship between genetic distance and geographic distance (Pearson correlation coefficient Mantel test, r = -0.003).
Palabras clave : Ucides occidentalis; 16S rRNA gene; mangrove swamp; genetic diversity.