Abstract

PERALES-ROSAS, Daniel et al. Detection of sugarcane yellow leaf virus and sugarcane mosaic virus in sorghum (Sorghum bicolor (L.) Moench) in the state of Morelos, México. Scientia Agropecuaria [online]. 2018, vol.9, n.3, pp.423-427. ISSN 2077-9917.  http://dx.doi.org/10.17268/sci.agropecu.2018.03.14.

The study was carried out in the months of June-July 2017, with the purpose of studying the current situation of the viruses associated with the cultivation of sorghum (Sorghum bicolor (L.) Moench). Twenty-one commercial sorghum plantation in Morelos state, were analyzed to detected Sugarcane yellow leaf virus (SCYLV) and Sugarcane mosaic virus (SCMV). The diagnosis was made, by means DAS-ELISA (double antibody) an immunoenzymatic test, that used a AGDIA kit (2017) and a reagent set of NANO Diagnostic (2017) "AC Diagnostic" with conjugated alkaline phosphatase, to detection of SCMV and SCYLV respectively. The results obtained evidenced, the presence of both viral diseases in the sorghum cultivation, in ten municipalities of Morelos state whit predominance of SCYLV. Positives samples to this virus with high absorbance (1.50 A° and 1.10 A°) on the municipalities of Amacuzac and Tequesquitengo, respectively were observed. While, only two positive samples of SCMV, in Puente de Ixtla and Yautepec were detected, but with low absorbance values respect to positive controls

Keywords : sorghum; virus; DAS-ELISA.

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