Resumo

CRUZADO-BRAVO, M.L.M et al. Biofilms of Staphylococcus spp. on stainless steel by milk and brain heart infusion broth as culture media. Scientia Agropecuaria [online]. 2018, vol.9, n.4, pp.485-491. ISSN 2077-9917.  http://dx.doi.org/10.17268/sci.agropecu.2018.04.03.

Staphylococcus spp. can adhere and produce biofilm on different contact surfaces. Thus, the aim of this work was to compare the biofilm production by Staphylococcus spp. on stainless steel using two culture media, milk Ultra High Temperatura (UHT) and Brain Heart Infusion broth (BHI). The bacterial count of adhered cells on steel was performed two times (12 and 24 hours) for both media evaluated with incubation at 25 °C. The bacterial counts of adhered cells were analyzed using ANOVA and Duncan's test for average comparison (p < 0.05). The biofilm formation of two strains (wild type strain and type strain) were observed applying Scanning electron microscopy (SEM). The bacterial counts were between 7 and 8 log CFU/cm2. Significant difference was found among the strains evaluated; however, no significant difference was described in times and culture media tested. It was showed by SEM that the wild type strain (S.a2) had considerable ability to biofilm production, which was expected due its results on bacterial counts. Thus, it can indicate milk offers appropriate conditions to biofilm formation of Staphylococcus spp. on stainless steel

Palavras-chave : Contact surface; culture media; bacterial biofilm; SEM.

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