Resumo

GUTIERREZ-ROSATI, Antonietta  e  GONZALES B, Patrick. Growth regulators for in vitro culture of three grapevine rootstocks (Vitis vinifera L.) used in the pisco industry. Scientia Agropecuaria [online]. 2019, vol.10, n.4, pp.461-468. ISSN 2077-9917.  http://dx.doi.org/10.17268/sci.agropecu.2019.04.02.

The objective of the study was to evaluate the use of growth regulators in culture media for three grapevine rootstock cultivars (Vitis vinifera L.) variety Harmony, Freedom and Paulsen used in the pisco industry. Benzylaminopurine (BAP) and naphthalenacetic acid (ANA), were tested as growth regulators in different concentrations and combinations, supplemented to the MS/2 culture medium. 21 treatments were analyzed for each cultivar. The variables evaluated were plant length, number of leaves, root length and other non-quantitative characteristics at 15 and 45 days after sowing. From the results obtained, it is concluded that the best media of propagation is MS/2 + ANA 0.5 ppm for Harmony; MS/2 + ANA 0.05 ppm for Freedom and MS/2 + ANA 0.075 ppm for Paulsen cultivar. Also, a genotype-dependent response was observed for the effects of auxin and cytokinin on the cuttings. However, all cuttings developed callous tissues when BAP was added to the media

Palavras-chave : naphthaleneacetic acid (ANA); benzylaminopurine (BAP); micropropagation; Harmony; Paulsen; Freedom.

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