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Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
Print version ISSN 2225-5109On-line version ISSN 2227-4731
Abstract
YACARINI-MARTINEZ, Antero Enrique; ARRIAGA-DEZA, Emma Vanesa and BELTRAN-ORBEGOSO, Raúl Antonio. Detection of Patotypes of Escherichia coli Strains Causing Childhood Diarrhea in Health Establishments of the Lambayeque-Peru Region, 2018. Rev. Cuerpo Med. HNAAA [online]. 2020, vol.13, n.3, pp.299-302. Epub Sep 30, 2020. ISSN 2225-5109. http://dx.doi.org/10.35434/rcmhnaaa.2020.133.741.
Objective:
To identify pathotypes of virulence genes of Escherichia coli from diarrheal samples of children under five years of age in the Lambayeque Region, in the period April-September 2018.
The study:
Descriptive and cross-sectional study. 75 E. coli strains from diarrheogenic samples were analyzed using real-time Multiplex PCR and primers for E. coli pathotype genes: daaD, aggR, eaeA, stx, ipaH and st.
Findings:
34.66% (26/75) of virulence genes were detected from the total isolates. The enterotoxigenic E. coli pathotype presented the highest frequency, 18.67% (14/75) of the total isolates. It was also detected that the enterotoxigenic E. coli pathotype appeared in 53.86% (14/26) of the total positive strains. Also, in 11.54% of positive strains (3/26) they presented more than one pathotype.
Conclusion:
Molecular epidemiology will help a better, adequate and timely diagnosis of childhood diarrhea in this vulnerable population.
Keywords : Escherichia coli; genes; Virulence factors; diarrhea.
