Abstract

LIMA JIMENEZ, Nelson Ramiro et al. In vitro propagation of Cinchona officinalis L from seeds. Rev. investig. Altoandin. [online]. 2018, vol.20, n.2, pp.169-178. ISSN 2313-2957.  http://dx.doi.org/10.18271/ria.2018.361.

ABTRACT Plant micropropagation is a useful tool for the conservation of threatened forest species with the use of in vitro culture techniques. The main objective was to study the in vitro propagation of Cinchona officinalis L from seeds. NaOCl was applied at different concentrations and immersion times for seed disinfection, medium MS (Murashige and Skoog) was supplemented with GA₃ for seed germination and for in vitro multiplication phase was used the combination of auxin and cytokinins in different concentrations. The treatments with NaOCl at 50 % for 5, 10 and 15 min were those that obtained the lowest percentage of contamination; the highest percentage of germination in the seeds was obtained with 1 mg/l of GA₃ and the best results in vitro multiplication were obtained with the combination of 0.2 NAA + 2.0 BAP. In vitro propagation protocols were generated in the species Cinchona officinalis L with the purpose of undertaking conservation programs and recovering native forest ecosystems of the southern region of Ecuador

Keywords : C. officinalis; in vitro; disinfection; germination; multiplication.

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