Abstract

AMESQUITA, Leticia et al. Chemopreventive effect of Caesalpinia spinosa (Fabaceae) against the genetic damage induced by cyclophosphamide in Rattus norvegicus. Arnaldoa [online]. 2018, vol.25, n.3, pp.953-960. ISSN 1815-8242.  http://dx.doi.org/10.22497/arnaldoa.253.25308.

The aim of the research was to evaluate the chemopreventive effect of Caesalpinia spinosa against the genetic damage induced by cyclophosphamide in polychromatic erythrocytes of Rattus norvegicus Holtzman strain, using the micronucleus (MN) test. We worked with groups of five individuals and randomly applied the treatments: a) negative control, water; b) C. spinosa, aqueous extract 20 mg/ml; c) C. spinosa-cyclophosphamide, 50 mg/kg/bw; d) positive control: cyclophosphamide 50 mg/kg/bw. The individuals were sacrificed and processed to obtain the cytological preparations. The analysis revealed that the frequency of MN in micronucleated polychromatic erythrocytes treated with cyclophosphamide was 8.81 ± 0.52, while the frequency of MN in the C. spinosa- cyclophophamide group was 5.53 ± 0.78. It was demonstrated that the aqueous extract of C. spinosa has a preventive effect by significantly decreasing the frequency of MN in individuals exposed to cyclophosphamide.

Keywords : Caesalpinia spinosa; cyclophosphamide; chemoprevention; Rattus norvegicus.

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