Abstract

LUNA CARDOSO, Pamela; SALIRROSAS, David  and  PRIETO, Zulita. Genetic stability in plants of Vitis vinifera cv. red globe, La Libertad, Peru, evaluated with microsatellites VVMD5 and VsZAG79 . Arnaldoa [online]. 2018, vol.25, n.3, pp.981-988. ISSN 1815-8242.  http://dx.doi.org/10.22497/arnaldoa.253.25310.

The aim of this work was to estimate the genetic variation in a crop of red globe variety (Vitis vinifera) by means of the microsatellites VVMD5 and VrZAG79. For this, plant leaves were obtained from San Jose camp at Chavimochic, La Libertad, Peru. Then, we proceeded with the extraction of DNA, the amplification by the polymerase chain reaction (PCR) and electrophoresis for the microsatellites VVMD5 and VrZag79. We observed monomorphic loci for both markers, the sizes of amplified fragments were of 213.6 and 239.7 bp for VVMD5 and of 242.2 bp for VrZAG79. As conclusion, the genotypes show stability during the replicas that were realized by vegetative cloning to obtain the plants from an initial genotype. Nevertheless, the evaluation of the genotypes will be necessary with additional markers.

Keywords : Vitis vinifera; grape; red globe; microsatellite markers.

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