Abstract

CHOLAN PACHECO, Karina; ZAVALETA ESPEJO, Gina; SALDANA JIMENEZ, José  and  BLAS CERDAN, Willian. Effect of hydroalcoholic extract of Caesalpinia spinosa (Fabaceae) on the growth of Salmonella typhi and Escherichia coli. Arnaldoa [online]. 2019, vol.26, n.2, pp.699-712. ISSN 1815-8242.  http://dx.doi.org/10.22497/arnaldoa.262.26212.

In the present investigation, the effect of the hydroalcoholic extract of Caesalpinia spinosa (Fabaceae) on the growth of Salmonella typhi and Escherichia coli was determined using a completely randomized block design. For which, a hydroalcoholic extract was prepared at 70° with mature and dry pods of C. spinosa. The evaluation was carried out using the modified Kirby Bauer method; instead of using disks, holes were used made with a sterile steel punch with a diameter of 7 mm. In each of the holes, 50 uL of the hydroalcoholic extract of C. spinosa was placed at concentrations of 200 mg/ml; 400 mg/ml and 800 mg/ml respectively; a positive control (ciprofloxacin) and a negative control (2 % dimethyl sulfoxide) were used, incubation at 37 ° C for 24 hours and finally the diameters (mm) of the inhibition zones were measured. The results show a directly proportional relationship between the increase in the concentration of the extract and the inhibition of the growth of both bacteria. It is observed that S. typhi has the largest inhibition diameter (29.33 mm) at the concentration of 800 mg/ml, while E. coli has an inhibition diameter of 24.44 mm at the same concentration. This is probably due to the antibacterial action of compounds such as tannins, quinones, phenols and flavonoids, gallotannins and gallic acid present in the extract. It is concluded that the hydroalcoholic extract of C. spinosa at different concentrations inhibits the growth of Salmonella typhi and Escherichia coli under laboratory conditions

Keywords : hydroalcoholic extract; Caesalpinia spinosa; Salmonella typhi; Escherichia coli; growth; inhibition.

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