Resumen

LINO NAVARRO, Mirko et al. antifungal response and PGPB properties of actinomycetes isolated from “vid” Vitis vinifera (Vitaceae) crops. Arnaldoa [online]. 2020, vol.27, n.2, pp.571-586. ISSN 1815-8242.  http://dx.doi.org/10.22497/arnaldoa.272.27209.

Plant growth-promoting bacteria (PGPB) are a tool that helps to have clean and sustainable agriculture as well as in the reduction of agrochemicals. 35 Actinomycetes were isolated from soil samples from vine, Ica crops. These 35 strains were characterized by their enzymatic ability to degrade different substrates such as oats, lecithin, starch, casein, tween 81 and carboxymethylcellulose. 18 actinobacteria strains were selected that had the best antagonistic response against Aspergillus sp. after being evaluated by bilayer technique in different culture media such as nutrient agar, plate count agar, potato dextrose agar and a new culture medium called ASMICO. To verify the PGPB capacity, phosphates solubilization, nitrogen fixation, siderophore production and acetic indole acid (AIA) production were qualitatively evaluated. 4 actinomycetes were selected with the best antagonism and with at least one of the PGPB characteristics. They then clashed against the phytopathogen Phytophthora cinnamomi which reduced their growth to 10 mm compared to 49 mm of negative control. Using ANOVA with the Statistica 10 software it was confirmed that the new ASMICO culture medium has the greatest effect and is significantly (p <0.05) different compared to the 3 other media used. It is concluded that actinomycetes isolated from Vine crops soils are a potential source to be used as producers of antifungal metabolites as well as PGPB.

Palabras clave : Actinomicetes; vid; antifungal; antagonism; PGPB.

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