Abstract

RAMOS OTINIANO, Cynthia Catheryne; HIDALGO RODRIGUEZ, José Ernesto Manuel; LEZAMA ASENCIO, Pedro Bernardo  and  CHAMAN MEDINA, Mercedes Elizabeth. Effects of Rhizophagus irregularis Schenk & Smith and NaCl on the electrophoretic pattern of “tomato” (Solanum lycopersicum L. (Solanaceae). Arnaldoa [online]. 2021, vol.28, n.2, pp.365-382. ISSN 1815-8242.  http://dx.doi.org/10.22497/arnaldoa.282.28207.

Salinity is an abiotic factor that produces a decrease in crop yields, plants respond by modifying their gene expression and inducing changes in protein synthesis. The objective was to determine the effect of Rhizophagus irregularis and NaCl on the electrophoretic pattern of tomato (Solanum lycopersicum L. (Solanaceae). Tomato plants were inoculated with R. irregularis (0, 1.5 and 3 g of inoculum) and treated with 0, 100 and 200 mM NaCl, for 30 days. Then, the total soluble protein content is quantified by the Bradford method and its electrophoretic pattern is evaluated by SDS- PAGE on leaves and roots. The total soluble protein content in the compliant leaves increases the amount of inoculum of R. irregularis, while in the adjusted conforming roots the concentration of NaCl increases; as for its electrophoretic pattern, in leaves, a 111 kDa protein appears due to the effect of NaCl and R. irregularis; in roots a protein of 73 kDa appears; In addition, changes in the intensity of some bands in leaves and roots were observed. Possibly these changes are consequences of the alteration of protein synthesis due to the ionic and osmotic imbalance generated by NaCl.

Keywords : tomato; salinity; Rhizophagus irregularis; electrophoretic pattern.

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