Abstract

SANCHEZ TISNADO, Denis Andree; SORIANO BERNILLA, Bertha Soledad; LUJAN VELASQUEZ, Manuela Natividad  and  JIMENEZ CORONADO, Marianela. Escherichia coli producing extended spectrum beta-lactamases, isolated in urine cultures of patients Cascas-La Libertad Hospital, Peru. Arnaldoa [online]. 2024, vol.31, n.1, pp.187-198.  Epub Apr 20, 2024. ISSN 1815-8242.  http://dx.doi.org/10.22497/arnaldoa.311.31110.

The prevalence of ESBL extended-spectrum beta-lactamase-producing Escherichia coli was determined in urine cultures of patients treated at the Cascas Hospital, La Libertad, Peru. 200 urine cultures were processed and sown on plates with Blood Agar and Agar MacConkey calibrated loop method, incubated at 37°C for 24 to 48 hours, cultures that presented colonies macroscopically similar to E. coli were selected, differential biochemical identification was performed. The plate diffusion method and the Jarlier method were applied for the detection of E. coli ESBL against Amoxicillin/Clavulanic Acid (20/10 μg), cefotaxime (30 μg), ceftazidime (30 μg), aztreonam (30 μg) and ceftriaxone (30 μg). 100 cultures positive for E. coli were obtained, of which 16 (16.0%) were ESBL, 5 (32.25%) came from men and 11 (68.75%) from women; The age group between 61 and 80 years had a higher prevalence for E. coli ESBL with 6 (37.5%) positive cultures; and the antimicrobials aztreonan and cefotaxime had a higher prevalence in ESBL E. coli with 8 (50%) positive cultures for each antimicrobial. It is concluded that the prevalence of E. coli producing extended-spectrum beta- lactamases in urine cultures from patients at the Hospital de Cascas - La Libertad in the period from January to July 2021 was 16.0%. With greater prevalence for the female gender, the age group between 61 to 80 years and for the antimicrobials of aztreonan and cefotaxime

Keywords : Escherichia coli; extended spectrum beta-lactamases; urine cultures.

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