Abstract

PARIONA-HUAPAYA, Delia; ZAVALETA, Amparo Iris; ACUNA, Gina Fiorella  and  ZAMUDIO-MALPARTIDA, Karin. In vitro prebiotic potential of the atomized extract of Swartzia polyphylla, Maytenus macrocarpa and Jatropha macrantha on Lactobacillus plantarum and Lactobacillus acidophilus. Manglar [online]. 2022, vol.19, n.4, pp.317-322.  Epub Dec 17, 2022. ISSN 1816-7667.  http://dx.doi.org/10.57188/manglar.2022.040.

The combination of prebiotics stimulates the growth of intestinal biota. Atomized extracts containing phenolic compounds promote the proliferation of some lactic acid bacteria (LAB). The objective of this research was to determine in vitro prebiotic potential of the atomized extract (AE) of Swartzia polyphylla (Cumaceba), Maytenus macrocarpa (Chuchuwasi) and Jatropha macrantha (Huanarpo male) on L. plantarum ATCC 14917 and L. acidophilus ATCC 4356. To do this, the bacteria were grown in the De Man Rogosa Sharp (MRS) media containing 1 and 2% (w/v) of AE in anaerobiosis at 37 °C for 48 h. Then, the cell count was performed in MRS agar, and the kinetic parameters and pH were measured. In addition, the resistance of these bacteria to simulated gastrointestinal conditions was assessed. The AE stimulated bacterial growth by maintaining a cell concentration greater than 10 x 106 for 48 h. The growth rates were 0.635 and 0.656 h-1 for L. plantarum as well as 0.391 and 0.516 h-1 for L. acidophilus in the MRS media containing 1% and 2% (w/v) of AE, respectively. The protective effect of the AE under simulated gastrointestinal conditions for L. plantarum and L. acidophilus was significant at pH 7.0 and 2.0, respectively. Overall, the AE presented prebiotic potential in vitro.

Keywords : Swartzia polyphylla; Maytenus macrocarpa; Jatropha macrantha; prebiotic; lactobacilli.

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