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Revista Peruana de Medicina Experimental y Salud Publica

versión impresa ISSN 1726-4634

Rev. perú. med. exp. salud publica vol.37 no.2 Lima abr./jun 2020

http://dx.doi.org/10.17843/rpmesp.2020.372.4984 

Brief report

Molecular diversity in pathogenic variants of Vibrio parahaemolyticus in Peru

Junior Caro-Castro, Biologist, Bachelor in Microbiology and Parasitology

Orson Mestanza, Biologist, Master in Bioinformatics

Willi Quino, Medical Technologist, Master in Microbiology

Ronnie G. Gavilán, Biologist, Doctor in Biochemistry and Molecular Biology

1 Instituto Nacional de Salud, Lima, Perú.

2 Universidad Nacional Mayor de San Marcos, Lima, Perú.

3 Escuela Profesional de Medicina Humana, Universidad Privada San Juan Bautista, Lima, Perú.

INTRODUCTION

The presence of pathogenic bacteria in the marine environment increases interest in food safety because of their potential to cause outbreaks. Among them, the Vibrio parahaemolyticus stands out, a halophilic gram-negative bacteria widely distributed in coastal ecosystems, whose serotyping depends on somatic (O) and capsular (K) antigens produced under various environmental conditions 1.

Interest in V. parahaemolyticus began many years ago, after it was found to be the causal agent of foodborne infections in an outbreak in Japan. Historically, V. parahaemolyticus has been responsible for 20-30% of cases of foodborne infection in Japan and other Asian countries 2. Peru has recorded significant outbreaks since 1997, which have been associated with climate changes that are part of the El Niño phenomenon. These climate changes alter marine ecological conditions, for example, increasing the rate of plankton abundance 3. Most of these reports associate thermostable direct hemolysin (TDH) with the virulence of V. parahaemolyticus 4.

Currently, the global prevalence and emergence of V. parahaemolyticus infection is increasing, underlining the need for adequate surveillance of this pathogen. Conventional microbiology is insufficient to determine pathogenic variants and their geographical distribution. In contrast, molecular epidemiology tools, such as the multilocus sequence typing (MLST), are proposed as new alternatives to study infectious diseases based on molecular strain differentiation. This scheme allows the rapid genotypic characterization of microorganisms, due to the development of a centralized database (PubMLST) that allows the comparison of different genetic variants called sequence types (ST) and the delineation of potential dispersion routes 5.

The objective of this study was to determine the genetic variants of pathogenic isolates of V. parahaemolyticus associated with human cases and seafood circulating in Peru during 1995-2017, using the MLST technique and in silico detection of virulence genes.

KEY MESSAGES

Motivation for the study: In view of the possible global emergence of pathogens causing gastrointestinal infections, strengthening molecular epidemiological surveillance of microorganisms such as V. parahaemolyticus will contribute to the timely detection and control of outbreaks.

Main findings: Fifteen different genotypes of V. parahaemolyticus were detected, three of which have already caused important outbreaks in Peru, while the other 12 have the potential to cause future epidemics due to their virulent nature.

Implications: To update information on the circulating V. parahaemolyticus genotypes in Peru until 2017, mainly prevalence and distribution through time.

THE STUDY

A total of 16 strains were submitted as V. parahaemolyticus from the collection of the National Reference Laboratory of Enteropathogens of the Instituto Nacional de Salud (INS) (Bioproject: PRJNA556706) and also 86 Peruvian genomes available in the NCBI database (http://www.ncbi.nlm.nih.gov) were included for MLST analysis (Table 1).

The strains were recovered in alkaline peptone water (Merck, Germany) at 37 °C for 68 hours. Subsequently, they were seeded by striae on bile-esculin citrate thiosulfate agar plates (Merck, Germany), and incubated at 37 °C for 18 to 24 hours. The genus Vibrio was confirmed using conventional biochemical tests, and the species V. parahaemolyticus by PCR for the presence of the toxR gene described by Kim et al. ( 6.

DNA extraction was performed using the DNeasy Blood &Tissue kit (Qiagen, Germany). DNA concentration and purity were evaluated by spectrophotometry (Denovix, USA). Sequencing libraries were developed using the Nextera XT kit (Illumina, USA), and genomic sequencing was performed using the MiSeq high throughput sequencer (Illumina, USA) 7. The quality of the sequences obtained was evaluated using FastQC v0.11.5. The sequences were assembled de novo using the A5-miseq pipeline 8. Identification of the genus and detection of contaminated contigs was carried out using the Kraken program 9.

The allelic profiles and the genomes’ STs obtained were assigned according to the information in the MLST data base for V. parahaemolyticus (http://pubmlst.org/vparahaemolyticus), using the MLST v2.10 program, based on the seven-locus scheme described for V. parahaemolyticus 5. Clonal complex assignment was performed using BioNumerics v7.5 (Applied Maths). Inclusion in a clonal complex (CC) was restricted to STs that shared at least 6 of the 7 alleles, while singletons were defined as STs that differed in two or more alleles from the other STs. With the same program a minimum spanning tree (MST) was generated showing the ST and the CC included in this work. In addition, a bar chart was constructed from the isolates studied using Infostat, to visualize genotypes by year of isolation and by number of samples.

The BLASTn tool was used to search for virulence factors of V. parahaemolyticus: the pathogenicity island type 7 (VpI-7) that internally contains the most common variant of TDH obtained from chromosome 2 of the RIMD genome 2210633 (access number: NC_004605.1) and TRH obtained from isolate AQ4299 (access number: LC271586.1), identifying as homologues those with <90% identity and <60% coverage of reference alignment. The code used for the annotation is available at http://github.com/OrsonMM/Blast-score-ratio-for-genomics. The results obtained were ordered in table format indicating the presence or absence of genes. All the sequences obtained during the study have been deposited in GenBank (Bioproject access number: PRJNA556706).

RESULTS

All 16 strains were confirmed as V. parahaemolyticus due to the presence of the ToxR gene. As for the genomic information, an average genomic size of 5.18 bp was obtained, composed by 80 contigs and a GC percentage of 45.2%.

A total of 102 genomes of Peruvian strains of clinical (97) and environmental (5) origin, isolated during 1995-2017, were analyzed (Table 1). The 102 genomes were classified into 15 different STs, which were grouped by year of isolation and origin of the isolate (Figure 1). It is observed that no isolates were obtained in 2004, 2010 and 2012.

Table 1 Table of Peruvian Vibrio parahaemolyticus data used in this study. 

UT: Untypified

Figure 1 Distribution of Vibrio parahaemolyticus by year of isolation, prepared with the InfoStat program. The lower legend indicates the color according to the type of sequence. 

The populational structure of the Peruvian V. parahaemolyticus strains (n=102), analyzed by MLST, can be visualized by means of a Minimum spanning tree graph (MST) (Figure 2). All strains belonging to the O3:K6 pandemic complex were grouped in the ST3 (n=53), representing 52% of the strains analyzed. In addition, clonal complex 345 (CC345) was identified, consisting of ST88 (n=3) and ST265 (n=9), both of serotype O4:K8, which differ at a single locus, with an isolation frequency of 11.8%. The remaining strains were included in 12 unrelated STs, while ST120 23.5% (n=24) and ST36 1.9% (n=2) were notable for being related to local or global outbreaks or epidemics (Annex 1).

Figure 2  Minimum spanning tree (MST) of 102 V. parahaemolyticus MLST allelic profiles included in this study designed with BioNumerics v7.5 software. The caption indicates each type of sequence, differentiated by color. Each circle represents a MLST genotype and the size is proportional to the number of strains included in each one. The branches connecting the circles indicate that they belong to the same clonal complex. 

From the 102 genomes analyzed, 89 isolates had the pathogenicity island, VpaI-7 (87.3%). Genes copies encoding TDH were found in 96 isolates (94.1%), the most frequent STs were ST3, ST36, ST88, ST120 and ST265. Genes for TRH were found only in ST36, ST64, ST65 and ST417, for a total of 6 isolates (5.9%). The results grouped by ST can be seen in Table 2, while the results per gene isolates composing VpaI-7 can be seen in Annex 2.

Table 2  In silico detection of V. parahaemolyticus virulence factors by sequence type 

Type of sequence n VpaI-7 tdhA tdhS trh
ST3 53 53 53 53 0
ST19 1 0 0 0 0
ST36 2 0 2 2 2
ST64 1 0 1 1 1
ST65 2 0 0 0 2
ST88 3 3 2 2 0
ST89 1 0 1 1 0
ST93 1 0 1 1 0
ST94 1 0 0 0 0
ST120 24 24 23 23 0
ST199 1 0 1 1 0
ST265 9 9 9 9 0
ST417 1 0 1 1 1
ST1169 1 0 1 1 0
ST1737 1 0 1 1 0
Total 102 89 96 96 6

n: number of isolates, VpaI-7: pathogenicity island 7, tdhA: thermostable direct haemolysin gene A, tdhS: thermostable direct haemolysin gene S, trh: TDH-related haemolysin gene

DISCUSSION

V. parahaemolyticus is a pathogen transmitted by high-demand food, yet little information is available on pathogenic variants and the temporal prevalence of its genotypes in Peru. This under-report of V. parahaemolyticus infections in the hospital setting is due to deficiencies in monitoring and research of foodborne diseases 10.

When analyzing the temporal prevalence of detected genotypes, the oldest isolates are found to correspond to the years 1995-1996, especially serotype O4:K8, but different genotypes: ST88 and ST265. Previous studies report this serotype since 1980, which caused sporadic cases and small outbreaks associated with raw seafood consumption; the highest prevalence was reported in 1983 ( 11. Molecular surveillance studies revealed China as the origin of this serotype, which is composed of several genotypes that have no clonal relationship with the serotype O3:K6, suggesting CC345may be an important clonal complex. In addition, comparative genomics analyses revealed that these isolates presented the secretion system regions type 3 (T3SS) and VpaI-7 12, which is consistent with what was found in this study.

In the distribution per year, the presence of ST3 stands out; and MST is the genotype with the highest number of sequenced isolates, from strains isolated between 1997 and 2017. All of them had the complete VpaI-7. The first Peruvian outbreak of ST3 occurred in 1997; however, it is known to have emerged in India in 1996, expanding to the American continent 13. This genotype belongs to the pandemic clonal complex CC3, which is distributed worldwide, and still is the dominant clone today 5. This clonal complex is the most studied genotype because it has the majority of VpaI reported for V. parahaemolyticus, being VpaI-7 the most important one, associated to cytotoxicity and enterotoxicity due to the presence of TDH and T3SS 14.

ST120 is the second group with the highest number of isolates in this study, obtained mostly during 2009, due to a serotype O3:K59 outbreak in the northern regions of the country 15. After the application of molecular surveillance by MLST it was defined to be ST120, which originated from China, and represents the third introduction of pathogenic populations of V. parahaemolyticus 16. In the results, the presence of this genotype during 2001, many years before its first report, is noteworthy, which would allow to reconsider when was the introduction of this genotype in Peru. Comparative genomic studies would be necessary to find differences between these isolates and those that caused the 2009 outbreak, which share the presence of VpaI-7.

Additionally, ST36, a small group of epidemiological importance within the Peruvian isolates analyzed, has its origin in the Pacific Northwest region of North America causing outbreaks in the United States and Canada 1. It has expanded since 2012 to rapidly reach other geographical areas such as northwestern Spain 17. Based on our results, the first strains belonging to this ST appeared in Peru in 2011, as part of the expansion of this clone in the Pacific 18. Although no outbreak of this genotype has been reported, it represents a latent epidemiological risk due to its pathogenic potential because of the presence of TDH and TRH. In this aspect, molecular surveillance by MLST can be applied for the timely tracking of these isolates and to curb outbreaks.

No information was found about other STs causing outbreaks or epidemics around the world. However, their pathogenic potential is not ruled out, due to the presence of genes encoding TDH or TRH, results that include genotypes such as ST1169 and ST1737.

Virulence factor analysis detected the presence of the genes encoding TDH in many clinical isolates, particularly in the genotypes with the highest number of isolates analyzed. It is known that the genes encoding TDH are mostly located within VpaI-7, so the presence of this region will have an impact on the increase in virulence of V. parahaemolyticus 19. However, the genetic deletion of the copies of the tdh gene or of the complete VpaI-7 does not determine the absence of virulence 14, which explains the detection of clinical strains with absence of TDH. On the other hand, genomes with TDH but without VpaI-7 were detected, which had already been described as TDH variants not associated with VpaI-7 20. Finally, the gene encoding TRH, which causes a similar effect to TDH, was found in a very small group of isolates.

In conclusion, ST3 is both temporally and quantitatively predominant in Peru, that is why still today, it is a genotype that generates risk in public health associated with the consumption of raw or semi-raw seafood. Notably, its pathogenic potential is due to the presence of VpaI-7, carrier of hemolysins. This, added to the underestimated epidemiological data, as well as the circulation of other pathogenic variants of these bacteria in the country, indicates that bigger efforts in molecular surveillance are needed, because this method is proving to be a powerful tool to detect and control outbreaks and infections.

Acknowledgements:

To Gustavo Bellido Mendoza for his invaluable technical support and to all the team of the National Reference Laboratory of Enteropathogens of the Instituto Nacional de Salud for the support provided during the realization of this work.

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Funding: The research was funded by Cienciactiva/FONDECYT (Agreement 145-2017-FONDECYT) and by the Instituto Nacional de Salud, Lima, Peru (OGITT: OI-0037-17).

Supplementary material: Available in the electronic version of the RPMESP.

Citation: Caro-Castro J, Mestanza O, Quino W, Gavilán RG. Molecular diversity in pathogenic variants of Vibrio parahaemolyticus in Peru. Rev Peru Med Exp Salud Pública. 2020;37(2):270-5. doi: https://doi.org/10.17843/rpmesp.2020.372.4984.

Received: November 20, 2019; Accepted: April 29, 2020

Correspondence to: Junior Caro Castro; Laboratorio de Referencia Nacional de Enteropatógenos, Instituto Nacional de Salud, Cápac Yupanqui 1400, Jesús María, Lima, Perú; juniorcaro12@hotmail.com.

Authors’ contribution:

JC, RG and WQ participated in the conception, hypothesis delineation and study design. JC and OM participated in the analysis, data interpretation and writing of the article. RG and OM participated in the critical review of the article. All approved the final version.

Conflicts of interest:

None.

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