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Revista de Investigaciones Altoandinas

versão On-line ISSN 2313-2957

Rev. investig. Altoandin. vol.23 no.4 Puno out./dic. 2021

http://dx.doi.org/10.18271/ria.2021.307 

Artículo Original

Presence of steroid hormones in Lake Titicaca and drinking water, Puno (Peru)

Presencia de hormonas esteroides en el lago Titicaca y agua potable, Puno (Perú)

Walter Alejandro Zamalloa Cuba1  * 
http://orcid.org/0000-0002-6817-0415

Sirleith Siomara Condori Canaza2 

Olivia Magaly Luque Vilca3 
http://orcid.org/0000-0002-9000-4624

Valdemar Luiz Tornisielo4 
http://orcid.org/0000-0003-2609-7333

Franz Zirena Vilca5 
http://orcid.org/0000-0002-4534-0733

1 Escuela Profesional de Ingeniería Química, Universidad Nacional del Altiplano, Av Floral S/N Puno, Perú.

2 Escuela Profesional de Ingeniería Química, Universidad Nacional del Altiplano, Av Floral S/N Puno, Perú.

3 Escuela Profesional de Ingeniería en Industrias Alimentarias, Universidad Nacional de Juliaca, Av. Nueva Zelandia 631, 21101 Juliaca, Perú.

4 Centro de Energía Nuclear na Agricultura, Laboratorio de Ecotoxicología, Avenida Centenario 303, 13416-903 Piracicaba, SP, Brasil.

5 Laboratorio de contaminantes orgánicos y ambiente del IINDEP de la Universidad Nacional de Moquegua, Urb Ciudad Jardín, Pacocha, Ilo, Perú.

Resumen

La presencia de hormonas esteroides en las aguas de los lagos provoca la contaminación de los ecosistemas acuáticos, lo que puede provocar alteraciones endocrinas en los organismos que los habitan. Además, muchas de estas aguas se depuran y distribuyen a las poblaciones ubicadas alrededor del lago; por tanto, estos efectos podrían repetirse en los seres humanos que consumen estas aguas. Este estudio reporta la presencia de residuos de hormonas esteroides en las aguas del interior de la bahía del lago Titicaca y agua potable en la ciudad de Puno (Perú). Para la preparación de la muestra se utilizó el método de extracción en fase sólida y los análisis se desarrollaron en un sistema HPLC-DAD, laboratorio CENA- USP- Brasil. Los resultados muestran concentraciones máximas de hormonas esteroides estrona (E1) 1,56, 17 β-estradiol (E2) 2,27, 17 α-etinilestradiol (EE2) 13. 88 ng L-1 respectivamente. Estas concentraciones varían en los diferentes puntos de monitoreo, y su presencia podría ocasionar efectos ecotoxicológicos a la biota acuática endémica que habita esta parte de este lago; al mismo tiempo también podrían afectar la salud de la población humana que consume esta agua.

Palabras clave: Cromatografía líquida; riesgo ambiental; contaminante emergente; Titicaca

Abstract

The presence of steroid hormones in lake waters causes contamination of aquatic ecosystems, which may cause endocrine alterations in the organisms that inhabit them. Moreover, many of these waters are purified and distributed to populations located around the lake. Therefore, these effects could be repeated in humans consuming the water. This study reports the presence of steroid hormone residues in the waters of the inner bay of Lake Titicaca and drinking water in the city of Puno (Peru). The solid phase extraction method was used for sample preparation, and the analyses were developed in an HPLC-DAD system. Results show maximum concentrations of steroid hormones estrone (E1) 1.56, 17 β- estradiol (E2) 2.27, 17 α- ethinylestradiol (EE2) 13. 88 ng L-1 respectively. These concentrations vary at the different monitoring points, and their presence could cause ecotoxicological effects to the endemic aquatic biota that inhabit this part of the lake. At the same time they also could affect the health of the human population that consumes this water.

Keywords: Liquid chromatography; environmental risk; emerging contaminant; Titicaca

Introduction

Hormones are increasingly used around the world (Torres et al., 2021), such as the hormone 17 α-ethinylestradiol (EE2), which is one of the compounds in birth control pills (González-Hernando et al., 2013) as well as livestock; therefore, they are excreted by humans and animals (Fent, 2015) and are capable of interfering with the endocrine system in vertebrates and invertebrates (Bovier et al., 2018). Hormones such as estrone (E1) and estradiol (E2) are endogenous estrogens catalyzed by cytochrome P450 (CYP) enzymes which have biological activity in the liver, tissues, extrahepatic target cells, and extrahepatic target cells (Zhu & Lee, 2005), producing metabolites such as estrogen hydroxylation with a similar structure but with different physiological effects after metabolism in vivo (Xu et al., 2021). All these compounds are eliminated through urine and human feces in different amounts, depending on age, health status, diet, pregnancy status of women and also through animal manure (González et al., 2020).

Hormones of natural and synthetic origin are also detected in the aquatic environment (Sacdal et al., 2020) as estrogens in inland water bodies, tributaries and wastewater treatment plant effluents (Deich et al., 2021), because of the ineffectiveness of conventional treatments (Torres et al., 2021). Runoff from farms and effluent from sewage treatment plants are affected by these pollutants (Sacdal et al., 2020), and lakes collecting discharges from urban areas are affected these types of pollutants. Concentrations of 85.15 ng L-1, 4.72 ng L-1 ,and 64.24 ng L-1 of E1, E2 and EE2, respectively, were found in the wastewater treatment plant of the city of Chascomús, Argentina (González et al., 2020), and its presence causes endocrine disruption that can affect aquatic organisms such as inhibition of gonadal growth and suppression of spermatogenesis (S. Wang et al., 2018). as it can also have adverse effects on the human body as an endocrine disruptor (Xu et al., 2021), It is therefore important to determine and monitor these emerging pollutants in water bodies (Katrina & Espino, 2020).

There is concern about emerging pollution in many lakes, which are important sources of water, considering that lakes are sometimes watershed drains and reservoirs that are altered by changes in land use and environmental conditions (Sacdal et al., 2020). The presence of hormones in lakes such as Laguna Lake, Philippines was found to be 0.03 and 0.30 ng L-1 of E1 and 0.36 ng L-1 of EE2 (Katrina & Espino, 2020), in Buyukcekmece Lake, Istanbul Turkey from <0.5 ng L-1 to 5.74 ng L-1 of E1, <0.5 ng L-1 to 10.2 ng L-1 of E2 and <0.5 ng L-1 to 11.7 ng L-1 of EE2 (Aydin & Talinli, 2013), while in East Lake, Wuhan China, nd to 9.62 ng L-1 E1, nd to 17.26 ng L-1 E2, and nd to 6.85 ng L-1 EE2 were found (J. Wang & Zhu, 2017), Also in Honghu Lake, China an average of 17.64 of E1, 7.26 ng L-1 of E2, and 17.73 ng L-1 respectively were found, as well as in Dongting Lake, China an average of 5.63 of E1, 10.32 of E2, 3.04 of EE2, 3.04 ng L-1 of EE2 (Yang et al., 2015).Space therefore, this study reports the presence of steroid hormone residues in the waters of the inner bay of Lake Titicaca and drinking water in the city of Puno.

The research focuses on the integral ecological system and contributes to science, specifically, to conservation of biodiversity and environmental studies (Escobar-Mamani, F., Branca, D., y Haller, A., 2020). In a transdisciplinary perspective and sustainable development (Haller, A. y Branca, D., 2020), environmental management is not isolated from the socio-economic, cultural, or physical characteristics of the environment.

The objective of this work was to evaluate the presence of steroid hormones in the waters of the interior bay of Lake Titicaca and drinking water of Puno by High-Efficiency Liquid Chromatography

Materials and methods

Sampling

The present study considered eight sampling points, collected in March 2020, at six points located in the inner bay of Lake Titicaca (distance 4 km), and two points located in houses supplied with water from the inner bay of Lake Titicaca (distance 3.5.km), (Figure 1).

Figure 1 Location, 6 sampling points, Lake Titicaca inland bay and two points located in houses supplied with water from the lake - Puno (Perú). 

Sampling was performed using a Niskin Teflon sampler, which is suitable for lake water sampling (Golnick et al., 2016), Once the sample was taken, it was transferred to a 1L amber glass bottle with a screw cap, which was previously sterilized; the samples were kept at a temperature below 4 °C, to avoid degradation by photolysis. They were then were transported in thermal boxes to a laboratory to proceed with the extraction process.

Sample preparation

HPLC grade water (Pura-Q. Brazil), HPLC grade methanol, Sigma-Aldrich sodium formate, and analytical standards for the hormones presented in Table 1 were used. Once in the laboratory, the samples were vacuum filtered with a 2.5 µm pore size paper filter to remove particulate matter from the sample. The hormone extraction process was carried out in the laboratory of the Universidad Nacional del Altiplano (Peru), by preconditioning the STRATA Polymeric Reversed Phase 500 mg/6 mL, Tubes, (SPE) solid-phase extraction cartridges were preconditioned with 5 mL of ultrapure water, followed by 5 mL of HPLC grade methanol, for each filtered sample (1L) and then dried, placed in cold chain and transported to the laboratories of CENA - USP -Brazil, where the analyses were carried out. Cartridges were rinsed with 5 mL of HPLC grade water at a flow rate of 1. 5 mL min-1, the elution process was developed using 10 mL of HPLC grade methanol and placed in tip test tubes; subsequent to this, the samples were dried in a turbovap at a temperature of 40 °C and with a constant flow of constant nitrogen at 12 PSI. Once the sample was almost dry, it was reconstituted with 1 mL of a 50 % HPLC grade methanol and 50 % ultrapure water solution, filtered and transferred to a vial for subsequent analysis by HPLC-DAD; this extraction method was previously validated by Hortense Torres, (2014) in the laboratory where the analyses of the present study were carried out.

Table 1 Characteristics of hormones used for standards. 

Hormone Purity % Supply Chemical Origin Molecular Formula Molecular Weight (g mol-1)
E1 99.0 Dr, Ehrenstorfer GmbH, Augsburg, Germany Natural hormone C18H24O3 270.37
17 alfa-EE2 98.5 Dr, Ehrenstorfer GmbH, Augsburg, Germany Synthetic hormone C20H24O2 296.40
17 beta- E2 99.0 Dr, Ehrenstorfer GmbH, Augsburg, Germany Natural hormone C12H24O2 272.38

Chromatographic analysis

Chromatographic analyses were performed on a liquid phase HPLC-DAD chromatograph (Agilent, model 1200), with an Agilent Zorbax Eclipse Plus C18 column (100 x 3 mm, 3.5 µm); the chromatographic conditions used are shown in Table 2. For the quantification of the hormones present in the samples, standard solutions of the hormone standards Estrone (E1), 17α ethinylestradiol (EE2) and 17 β-estradiol (E2) were prepared in triplicate in concentrations of 0.5, 1, 3, 6 and 10 ug L-1 respectively, these solutions were prepared in HPLC-grade methanol, the mobile phase consisted of 20:80 (V/V) (A/B) (mobile phase A ultrapure water with 5 mmol sodium formate and mobile phase B was HPLC grade methanol) used in isocratic mode at a flow rate of 1 mL-1, with an injection volume of 10 µL for all samples and standards, method used which has been developed and validated by (Hortense Torres, 2014).

Table 2 Chromatographic conditions for the identification and quantification of hormones. 

Parameter Conditions
Flow 1 mL min-1
Wavelength 282 nm
Analytical run time 15 minutos
Injection volume 10 µL
Column temperature 30°C
DAD Detector 282 nm

Results

Hormone residues were found at the monitoring points considered in this study; the results are shown in Table 3.

Table 3 Steroid hormone concentration expressed in ng L-1 at the sampling points. 

Hormones P1 P2 P3 P4 P5 P6 P7 P8
17 β-estradiol E2 1.61 2.27 0.90 1.12 1.50 1.12 1.53 1.21
17 α-etinilestradiol EE2 1.38 1.62 5.76 5.80 8.97 13.88 1.03 0.81
Estrone E1 0.76 0.74 0.83 0.72 0.75 1.56 0.80 0.77

This presence could be attributed to the fact that near the inner bay of this lake there is an oxidation lagoon, which is inefficient in the degradation and/or removal of hormone residues and therefore, the poorly-treated water is being discharged into the lake (Torres et al., 2021). Thus at point 6, the main point of outflow (oxidation lagoon) presents concentrations of 17 α-ethinylestradiol (EE2) 13.88 ng L-1 and estrone (E1) 1.56 ng L-1, as well as 17 β-estradiol (E2) 1. 12 ng L-1, and as it travels until it reaches point P1- Chimú- (water intake for purification) these concentrations decrease. Point 1. concentrations of 17 α-ethinylestradiol (EE2) 1.38 ng L-1 and estrone (E1) 0.76 ng L-1, as well as 17 β-estradiol (E2) 1.61 ng L-. drinking water point 7 with 1.03 ngL-1 of EE2, 1.03 ngL-1 of E2, 1.56 ngL-1 E1 and point 8 with 0.81 ngL-1 of EE2, 1.21 ngL-1 of E2 and 0.77 ngL-1 of E1. It is shown in Figure 2.

Figure 2 Graphic representation of the concentration of hormones Vs Study Points P6 a P1 Lake water, P7 a P8 drinking water -Puno. 

Discussion

This is due to their dispersion in water and depth, considering that these chemical compounds have lipophilic properties (Langenbach, 2013), low vapor pressure between 2.3 x 10-10 and 6.7 x 10-15 mm Hg, and also present log Kow values of the steroids of 2.81 for estriol and 3.94 for 17β-estradiol, while the steroid 7α-ethinylestradiol has a Log Kow value of 4.15. a characteristic of these compounds that allows them not to be soluble in water so it is expected to sediment and is a cause for reduced concentrations in the aqueous phase (Adeel et al., 2017), as well as the effect of sunlight exposure that produces a phototransformation of E2 and EE2 with a yield of 0.06 mol einstein-1 at 254 nm irradiance. (Mazellier et al., 2008).

The concentration of 17 β-estradiol E2 at point 1 was 1.61 ng L-1, this is lower than that found in Lake Luoma, China which ranged from 2.52 ng L-1 to 21.82 ng L-1, mean concentration of 9.41 ng L-1 (Dan Liu et al., 2017), and similar to that found in Buyukcekmece lake, Istanbul (Turkey) which ranged from <0.5-10.2 ng L-1 (Aydin & Talinli, 2013) these values are related to population density. The presence of these compounds in the ecosystem of the lake's inner bay could have toxicological effects on the endemic species that inhabit this ecosystem. 17 β-estradiol (E2) is capable of producing the oxidation of macromolecules, generating the formation of the superoxide radical anion (Cavalieri et al., 2000), which causes loss of physicochemical properties that may result in cell death upon prolonged exposure, thus at 2 μg L-1 of 17 β-estradiol affects several physiological processes, including the spliceosome, phototransduction, amino sugar and nuclear sugar metabolism, hypotaurine metabolism and the renin-angiotensin system. It was evidenced that exposure of O. curvinotus juveniles to increasing concentrations of 17 β-estradiol (2 ng L-1, 20 ng L-1, 200 ng L-1, and 2 μg L-1) leads to a significant increase in the mRNA expression of vitellogenins (VTGS) and choriogenins (CHGS) (Dong et al., 2020).

The concentration 17 α-ethinylestradiol EE2 at point 1 was 1.38 ng L-1, which is lower than that found in Lake Luoma, China (4.25 ng L-1 to 12.85 ng L-1; mean concentration of 7.97 ng L-1) (Dan Liu et al., 2017). However, at point 6 (outflow) it was 13.88 ng L-1, lower than that found in wastewater and revert to the Chascomús lagoon, Argentina of 64.24 ng L-1 and 47.55 ng L-1 (at 200 m). (González et al., 2020), This difference could be attributed to the population density that generates domestic wastewater, sewage effluents, aquaculture, runoff from livestock farming (Zhou et al., 2016), industrial waters (Jia et al., 2019). Likewise, 17-α-ethinyl-estradiol is a non-translocating and non-competitive inhibitor of proton-coupled amino acids (Nielsen et al., 2021) being capable of inducing alterations in the endocrine system in living organisms at concentrations in the water below 1 ng L-1 (Hansen et al., 1998; Purdom et al., 1994).

The presence of Estrone E1 at point 1 was 0.76 and is higher than that reported in surface waters of Laguna Lake, Philippines (0.03 and 0.30 ng L-1) which is used for drinking water production (Katrina & Espino, 2020), and the Langat River is also used as a source of drinking water in Malaysia (0.09-0.18 ng L-1; mean of 0.12 ng L-1 ± 0.04) (Wee et al., 2019). At point 6 it was 1.12 ng L-1, lower than reported in wastewater and discharged to the Chascomús lagoon, Argentina of 85.15 ng L-1, 56.11 ng L-1, and 1.54 (at 300 m upstream) (González et al., 2020). Estrone is converted to estriol by natural processes in surface waters (Aydin & Talinli, 2013) while high concentrations of E1 (>10 mg L-1) significantly decrease the productivity of extracellular polymeric substances and metabolic activity (Zhang et al., 2021).

Several studies have shown abnormal development of fish in water with hormone residues, such as the feminization of male fish, affecting reproduction and inducing vitellogenin production (Torres et al., 2021), growth of certain aquatic organisms (Sacdal et al., 2020), Therefore, it is important to consider toxicity concentrations in the life of the fish, taking into account the lipophilic nature of the hormones that collaborate with bioaccumulation; in Carassius auratus fish after 72 hours of dietary exposure containing 100 ng g-1 of EE2, the concentration was 1.7 ng g-1 ±0.29 (Al-Ansari et al., 2011).

However, the presence of hormone residues at points 7 and 8 (drinking water), raises concerns about potential human exposure to endocrine disrupting compounds through dietary intake of food and especially the drinking water supply. (Wee et al., 2019). Hormone residues were within the range found in treated waters in the northern, central and southern regions of Taiwan E1 ND - 2.3 ng L-1, E2 ND - 4.8ng L-1, and EE2 ND - 3.8 ng L-1 (Pai et al., 2020); estrogens 17-β-estradiol (E2) and 17-α-ethinylestradiol (EE2) are considered to be highly endocrine disrupting agents and have been included in the EU monitoring list on emerging aquatic pollutants due to their presence in animal tissues and fluids (Barreiros et al., 2016).

Conclusions

The waters of the inner bay of Lake Titicaca and drinking water whose main source comes from this bay, show concentrations of steroid hormones: estrone (E1) 1.56, 17 β-estradiol (E2) 2.27, 17 α- ethinylestradiol (EE2) 13. 88 ng L-1 respectively,

These concentrations vary in the different monitoring points as they move away from the main emission point (oxidation lagoon); the presence of these contaminants could cause ecotoxicological effects to the endemic aquatic biota that inhabit this lake, and at the same time it would be affecting the health of the population around the lake since they consume water from this lake.

The research focuses on the integral ecological system and contributes to science, specifically. Conservation of biodiversity, and environmental studies. In a transdisciplinary perspective and sustainable development, not isolated from the socioeconomic, cultural or physical characteristics of the environment.

Acknowledgements

To the Special Binational Lake Titicaca Project (PEBLT), for logistical support taking samples from Lake Titicaca. To the Nuclear Energy Center for Agriculture (CENA) -USP-Brazil - for the use of the HPLC-DAD equipment.

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Recibido: 24 de Mayo de 2021; Aprobado: 22 de Septiembre de 2021

*Autor correspondiente: zamalloacuba1@hotmail.com

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